CENTRAL NEUROCHEMISTRY SERVICE

中央神经化学服务

• 批准号: 3968502
• 负责人: W Z POTTER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3968502
• 关键词: 3 methoxy 4 hydroxyphenylethyleneglycol; blood chemistry; cerebrospinal fluid; clinical chemistry; dopamine; epinephrine; gas chromatography mass spectrometry; high performance liquid chromatography; homovanillate; human tissue; hydroxyindoleacetate; neurochemistry; neurotransmitter metabolism; norepinephrine; normetanephrine; serotonin; urinalysis

The Central Neurochemistry Service functions as a centralized laboratory for analysis of neurotransmitters and metabolites in body fluids collected within the intramural program. Routine analyses include norepinephrine in plasma, urine and CSF, epinephrine in urine and plasma, dopamine and dopamine sulfate in urine, plasma and CSF, catecholamine metabolites, HVA, MHPG and DOPAC in CSF, plasma and urine, serotonin in platelets and platelet poor plasma and 5HIAA in plasma, CSF and urine. GC-MS assays are used for total urinary norepinephrine, epinephrine and dopamine and urinary VMA, MHPG and normetanephrine. HPLC with electrochemical detection is used for other analyses. Modifications of existing procedures allow simultaneous determination of HVA, 5HIAA and MHPG in plasma. This allows simultaneous observation of drug and/or diurnal effects on metabolites of three different neurotransmitter systems. Over 8500 assays were performed on over 6000 samples which were processed.

中央神经化学服务中心作为一个集中实验室 用于分析收集的体液中的神经递质和代谢物 在校内计划内。 常规分析包括去甲肾上腺素 血浆、尿液和脑脊液、尿液和血浆中的肾上腺素、多巴胺和 尿液、血浆和脑脊液中的硫酸多巴胺、儿茶酚胺代谢物、HVA、 脑脊液、血浆和尿液中的 MHPG 和 DOPAC，血小板和血清中的血清素 血浆、脑脊液和尿液中含有贫血小板血浆和 5HIAA。 GC-MS 检测是 用于总尿去甲肾上腺素、肾上腺素和多巴胺以及尿 VMA、MHPG 和去甲变肾上腺素。 使用带有电化学检测的 HPLC 用于其他分析。 对现有程序的修改允许 同时测定血浆中的HVA、5HIAA和MHPG。 这允许 同时观察药物和/或昼夜对代谢物的影响 三种不同的神经递质系统。 进行了超过 8500 次检测 处理了 6000 多个样品。