MACROMOLECULAR DYNAMICS AND ASSEMBLY REACTIONS

大分子动力学和组装反应

• 批准号: 4689604
• 负责人: J HOFRICHTER
• 金额: --
• 依托单位: NAT INST OF ARTHRITIS, DIABETES, DIGESTIVE & KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4689604
• 关键词: adenosine triphosphate; atomic absorption spectrometry; birefringences; carbon monoxide; chemical association; chemical reaction; cobalt; computer simulation; dichroism; hemoglobin Ss; hemoprotein structure; human tissue; iron; laser spectrometry; light scattering; mathematical model; microspectrophotometry; orphan disease /drug; photolysis; polymerization; polymers; sickle cell anemia; solvents

Transient spectroscopy is used to study the kinetics of conformational changes in macromolecules subsequent to excitation with a pulsed laser. Changes in both the tertiary and quaternary structure of hemoglobin have been observed following the photodissociation of carbon monoxide from the hemes. Steady state photodissociation of carbon monoxide from hemoglobin S is used to study the nucleation-controlled kinetics of the assembly of deoxy hemoglobin S into polymers. This technique has recently been modified by adding an additional laser beam which can be used to prepare a partially saturated sample by photolysis of CO hemoglobin S or to probe the kinetics of the sample by modulation techniques and a pump and mixer system which permits cells to be equilibrated with solutions of known oxygen tension. A second, high intensity beam can then be used to remove all of the ligands and measure the polymerization kinetics of the resulting deoxy hemoglobin S sample. We have begun to use this system to explore the binding of CO to hemoglobin in the photo stationary state produced at different levels of photolysis and to measure ligand binding curves to single sickle cells.

瞬态光谱法用于研究构象的动力学 用脉冲激光激发后的大分子的变化。 血红蛋白的第三和第四纪结构的变化 在从 下摆。 使用血红蛋白S的一氧化碳的稳态光解离 研究脱氧组装的成核控制动力学 血红蛋白S成聚合物。 该技术最近已通过 添加额外的激光束，可用于准备部分 通过CO血红蛋白S的光解或探测动力学的饱和样品 通过调制技术和泵和混合系统的样品的样本 允许用已知氧张力的溶液平衡细胞。 一个 其次，然后可以使用高强度梁去除所有配体 并测量所得脱氧血红蛋白的聚合动力学 样本。 我们已经开始使用该系统探索CO与血红蛋白的结合 在不同水平的光解的光固态中 并测量配体结合曲线与单镰刀细胞。