MACROMOLECULAR DYNAMICS AND ASSEMBLY REACTIONS

大分子动力学和组装反应

• 批准号: 5201947
• 负责人: J HOFRICHTER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5201947
• 关键词: biophysics; blood disorder chemotherapy; carbon monoxide; chemical association; chemical binding; chemical kinetics; chemical models; conformation; cytochrome c; heme; hemoglobin Ss; hemoprotein structure; hydroxyurea; laser spectrometry; molecular dynamics; myoglobin; photochemistry; photolysis; polymerization; protein folding; sickle cell anemia; sickling inhibitor; time resolved data; viscosity

Time-resolved absorption and fluorescence spectroscopy are used to study the dynamics of protein structural changes subsequent to rapid mixing or excitation with short laser pulses. Molecular models for the protein dynamics are used to fit and interpret the measured data. A. We have succeeded in implementing two new techniques which can be used to study the kinetics of a wide variety of processes, a rapid mixing technique which, in principle, is capable of initiating reactions within 10 microseconds and a laser temperature jump experiment in which water is heated by an infrared laser pulse. B. The dynamics of short-lived states on the folding pathway of cytochrome c have been investigated using continuous-flow mixing and fluorescence quenching to monitor the tryptophan-heme distance. The most rapid phase observed occurs at about 100 microseconds, followed by a decay in fluorescence intensity which includes at least 4 additional exponential relaxations.

时间分辨吸收和荧光光谱用于研究 快速混合后蛋白质结构变化的动态或 用短激光脉冲激发。蛋白质的分子模型 动力学用于拟合和解释测量数据。 A. 我们已经成功实现了两种可以使用的新技术 为了研究各种过程的动力学，快速混合 原则上，能够在内部引发反应的技术 10 微秒和激光温度跳跃实验，其中水 由红外激光脉冲加热。 B. 细胞色素折叠途径短暂状态的动力学 c 已使用连续流动混合和荧光进行了研究 淬灭以监测色氨酸-血红素距离。最快速阶段 观察到的现象发生在大约 100 微秒，随后衰减 荧光强度，其中至少包括 4 个附加指数 放松。