BASIC STUDIES OF MYCOPLASMAS

支原体的基础研究

• 批准号: 4688331
• 负责人: J G TULLY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4688331
• 关键词: Mycoplasma; Mycoplasma pneumoniae; bacterial cytopathogenic effect; bacterial toxins; disease /disorder model; epidemiology; host organism interaction; human subject; immunofluorescence technique; latent virus infection; membrane activity; molecular biology; prokaryote; serotyping; tissue /cell culture; urethritis; virulence

These efforts cover both basic and applied aspects of mycoplasmas and related wall-free prokaryotes (mollicutes), including their molecular biology, membrane and cellular components involved in attachment, virulence, or immunological interrelationships, and their possible role in human disease or diseases of uncertain etiology. Current efforts involve the characterization of a group of sterol non-requiring mollicutes (genus Acholeplasma) isolated from variety of human tissue sites and from other environmental origins. A strain of Acholeplasma oculi has been identified in the amniotic fluid of a patient undergoing aminocentesis, and a strain of Acholeplasma laidlawii has been identified in the tissues of a stillborn infant. We have also characterized ten other acholeplasmas recovered from a variety of insect and plant origins. Most of these mollicutes have been found to be serologically distinct from all other acholeplasmas, but have the general features (no serum requirement for growth, genome size of 1 x 1\billion daltons, G+C value of 30-35 mole%, etc.) of acholeplasmas. Further efforts to understand the ecology of these mollicutes and their occurrence in human tissues appears justified. Additional work on identification of the attachment protein in Mycoplasma genitalium suggests that a 143 K protein is involved in adherence of the organism to human tissues, and this protein can be separated from the 165 K protein (P1) identified as the main attachment moiety in Mycoplasma pneumoniae. Antibody to the 143 K protein has been identified in the serum of chimpanzees following an experimental genital tract challenge with Mycoplasma genitalium.

这些工作涵盖了支原体的基础和应用方面 相关的无壁原核生物（软体动物），包括它们的分子 生物学，参与附着的膜和细胞成分， 毒力或免疫学相互关系及其可能的作用 人类疾病或病因不明的疾病。 目前的努力包括 一组不需要甾醇的软体动物（属 无胆原体）从多种人体组织部位和其他部位分离 环境起源。 已鉴定出一种眼无胆碱原体菌株 在接受氨基穿刺术的患者的羊水中，以及菌株 在死产婴儿的组织中发现了莱氏无胆原体 婴儿。 我们还鉴定了从其中回收的其他 10 种胆原体。 各种昆虫和植物的起源。 大多数这些软体动物已 发现在血清学上与所有其他胆原体不同，但 一般特征（生长不需要血清，基因组大小为 1 x 1\十亿道尔顿，G+C 值为 30-35 摩尔％等）胆原体。 进一步努力了解这些软体动物的生态学及其 人体组织中的发生似乎是合理的。 额外工作 生殖支原体中附着蛋白的鉴定表明 143 K 蛋白质参与生物体与人类的粘附 组织中，该蛋白可与 165 K 蛋白 (P1) 分离 被鉴定为肺炎支原体的主要附着部分。 143 K 蛋白抗体已在血清中鉴定出 黑猩猩在接受实验性生殖道挑战后 生殖支原体。