BASIC STUDIES OF MYCOPLASMAS

支原体的基础研究

• 批准号: 3768717
• 负责人: J G TULLY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3768717
• 关键词: AIDS; HIV infections; Mycoplasma; Mycoplasma pneumoniae; bacterial cytopathogenic effect; genetic strain; host organism interaction; human tissue; immunofluorescence technique; microorganism classification; microorganism culture; polyarthritis; serology /serodiagnosis; species difference

The role of Mycoplasma genitalium in human disease has been difficult to define. The organism is frequently mixed with M. pneumoniae and these two organisms share considerable immunologic reactivity. Thus, conventional complement-fixation tests or new immunosorbent assays for M. pneumonia infections can not differentiate serologic responses to each organism. We now have also identified mixed M. genitalium/M. pneumoniae strains in the synovial fluid of a patient with polyarthritis following primary pneumonia infection, suggesting that M. genitalium can become disseminated from either urogenital or oropharynx sites. We have currently defined an indirect immuno-fluorescence assay that will accurately distinguish colonies of each organism on agar plates when mixed strains are grown in culture. This test is being applied to specimens being compared in culture versus direct testing with a variety of molecular probes. Collaborative studies have also established that animal mycoplasmas (M. arginini) can induce severe and fatal infections in immunocompromised patients. The inability of antibiotic drugs to control such infections is related to the mycoplasmastatic activity of known antibiotics and the contribution of an intact humoral and cell mediated defense in the host. Taxonomic characterization of a new mycoplasma from the urine of both HIV- positive and AIDS patients has been carried out in collaborative studies. The high cytopathogenicity for a variety of eukaryotic cells appears related to the unique attachment structure on the organism and the ability of the organism to induce intra-cellular infections. The organism has been designated Mycoplasma penetrans.

生殖支原体在人类疾病中的作用一直难以确定 定义。 该微生物经常与肺炎支原体混合，而这两种微生物 生物体具有相当大的免疫反应性。 因此，传统的 肺炎支原体的补体结合试验或新的免疫吸附试验 感染不能区分对每种生物体的血清学反应。 我们 现在还鉴定出了混合生殖支原体/M. genitalium。肺炎菌株在 原发性肺炎后多关节炎患者的滑液 感染，表明生殖支原体可以从 泌尿生殖或口咽部位。 我们目前定义了一个 间接免疫荧光分析可准确区分 当混合菌株在琼脂平板上生长时，每种生物体的菌落 文化。 该测试适用于比较的样本 培养与使用各种分子探针的直接测试。 合作研究还证实动物支原体（M. arginini）可在免疫功能低下者中引起严重和致命的感染 患者。 抗生素药物无法控制此类感染 与已知抗生素的抑支原体活性有关 宿主中完整的体液和细胞介导的防御的贡献。 HIV-2 尿液中一种新支原体的分类学特征 阳性者和艾滋病患者已开展合作研究。 对多种真核细胞表现出高致细胞致病性 与生物体上独特的附着结构和能力有关 生物体诱导细胞内感染。 该生物体具有 被命名为穿透性支原体。