BASIC STUDIES OF MYCOPLASMAS

支原体的基础研究

• 批准号: 3818089
• 负责人: J G TULLY
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818089
• 关键词: Mycoplasma; Mycoplasma pneumoniae; Mycoplasmatales; antiserum; bacterial disease; bacterial genetics; bacterial proteins; binding proteins; host organism interaction; human subject; molecular biology; nomenclature; nucleic acid hybridization; nucleic acid probes; reproductive system; serotyping; urinary tract infection

Recent efforts have continued in characterization of the biological and molecular features of Mycoplasma genitalium, in anticipation that this information will lead to development of improved methods in detection of the organism and more definite confirmation of its role in human disease. Mycoplasma genitalium, first isolated from the urethra of two men with nongonococcal urethritis in our laboratory in 1980, has now been found retrospectively in throat specimens of military recruits participating in an inactivated M. pneumoniae vaccine field trial in 1974-75. Three of sixteen preserved throat isolates, previously identified as M. pneumoniae, have now been shown to be mixtures of M. pneumoniae and M. genitalium. Purification of these mixed mycoplasms by selection of single colony isolates clearly confirmed the presence of M. genitalium. Identification of M. genitalium was based upon the occurrence of species-specific 140 kDa protein adhesin in these isolates and their extensive serologic reactivity to a M. genitalium antiserum. The frequent occurrence of both M. genitalium and M. pneumoniae in a number of these throat specimens, in combination with shared serologic reactions between these two mycoplasms, suggests the likelihood that M. genitalium strains might be easily missed in current diagnostic laboratory techniques. What role M. genitalium might have in human respiratory disease remains to determined. Further studies on other mollicutes, primarily of insect orgin, have led to discription of new species of Acholeplasma, the isolation of acholeplasmas from human amniotic fluid, revised reclassification of helical mollicutes (spiroplasms), and an enlarging group of other mollicutes that have been difficult to assign to established taxonomic groups.

最近在生物表征方面继续努力 和预期的生殖支原体的分子特征 这些信息将导致改进方法的开发 检测生物体并更明确地确认其 在人类疾病中的作用。 生殖支原体，首先分离自 我们的两名患有非淋菌性尿道炎的男性的尿道 1980年实验室检查，现已在喉咙中回顾性发现 参加灭活 M 的新兵样本。 1974-75 年肺炎疫苗现场试验。 十六个中的三个 保留的咽喉分离株，之前被鉴定为肺炎支原体， 现在已被证明是肺炎支原体和肺炎支原体的混合物。 生殖器。 通过选择纯化这些混合支原体 单菌落分离株清楚地证实了 M 的存在。 生殖器。 生殖支原体的鉴定基于 物种特异性 140 kDa 蛋白粘附素的出现 分离株及其对分枝杆菌的广泛血清学反应。 生殖器抗血清。 两者M的出现频率都很高。 许多咽喉标本中含有生殖器和肺炎支原体， 结合这两者之间共同的血清学反应 支原体，表明生殖支原体菌株的可能性 在当前的诊断实验室技术中可能很容易被忽略。 生殖支原体在人类呼吸道疾病中可能发挥什么作用 仍有待确定。 对其他软体动物的进一步研究， 主要源自昆虫，导致了新物种的描述 胆原体的分离，从人羊膜中分离胆原体 流体，螺旋软体的修订重新分类 （螺原体），以及一个不断扩大的其他软体动物群体 很难分配到已建立的分类群。