Development of leucocyte cell lines for immunological research in teleost fish

用于硬骨鱼免疫学研究的白细胞系的开发

• 批准号: G0800725/1
• 负责人: Jun Zou
• 金额: $ 32.43万
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0800725%2F1
• 关键词: Development; leucocyte; cell; lines; immunological

White blood cells, or leucocytes, are the cell that defence the body from infections and provide disease resistance. Research into the function of leucocytes is greatly aided in mammals by the availability of many leucocyte cell lines, that allow a lot of research to be performed using culture systems without the need to harvest tissues and cells from animals. These developments have been possible in large part by the discovery of many leucocyte growth factors, that can be produced using molecular methodologies, and added to the cultures as growth supplements. In this proposal we aim to develop leucocyte cell lines for fish, due to increasing use of fish by researchers, partly as a consequence of the major expansion of aquaculture and its associated needs (eg fish health issues). The applicants are internationally renown for their work on the fish equivalents of the leucocyte growth factors, and so can produce relevant proteins to encourage leucocytes to grow in vitro. Several different approaches will be taken to generate the cell lines, and some preliminary characterisation is proposed, so that they will quickly become a valuable resource for researchers. In addition to a reduction in the numbers of fish being used, a reduction in fish suffering should also follow this development, since the cell lines will also allow preliminary in vitro testing of compounds and chemicals to see if they affect leucocyte function, before testing in live fish.

白细胞或白细胞是保护身体免受感染并提供抗病能力的细胞。在哺乳动物中，许多白细胞细胞系的可用性极大地帮助了对白细胞功能的研究，这些细胞系允许使用培养系统进行大量研究，而无需从动物身上收获组织和细胞。这些进展在很大程度上是由于许多白细胞生长因子的发现而成为可能，这些生长因子可以使用分子方法生产，并作为生长补充剂添加到培养物中。在本提案中，我们的目标是开发鱼类的白细胞细胞系，因为研究人员越来越多地使用鱼类，部分原因是水产养殖及其相关需求（例如鱼类健康问题）的大幅扩张。申请人因其在白细胞生长因子的鱼类等同物方面的研究而享誉国际，因此可以生产相关蛋白质以促进白细胞在体外生长。将采用几种不同的方法来生成细胞系，并提出一些初步表征，以便它们很快成为研究人员的宝贵资源。除了减少使用的鱼的数量之外，这种发展也应该减少鱼的痛苦，因为细胞系还可以在进行测试之前对化合物和化学物质进行初步的体外测试，看看它们是否影响白细胞功能。活鱼。