MOLECULAR AND DEVELOPMENTAL GENETICS OF DROSOPHILA

果蝇的分子和发育遗传学

• 批准号: 3096105
• 负责人: FOTIS C KAFATOS
• 金额: $ 83.29万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3096105
• 关键词: developmental genetics; genetic manipulation

We are involved in studying the structure and regulation of eukaryotic genes, using Drosophila as an experimental object. Although we come from diverse backgrounds (genetics, molecular biology, developmental biology, population genetics, cell biology), we intersect in our common interest: to use the genetically most favorable higher organism, Drosophila, and the current techniques of molecular and cellular biology, to illuminate how the genes of higher organisms are organized, evolve, and are regulated during development and during the cell cycle. Indeed, a strength of our group is that it represents complementary and yet overlapping approaches. In the context of the program project, we intend to: (a) Analyze by molecular and genetic means the mechanisms regulating the orderly production of chorion proteins during oogenesis. (b) Investigate by molecular and genetic means the processes controlling transvection, position effects and somatic chromosome pairing. (c) Study genetic polymorphism by sequencing the alcohol dehydrogenase and xanthine dehydrogenase genes of isogenic lines derived from natural population of Drosophila pseudoobscura. (d) Characterize RNA Polymerase II mutants of Drosophila, and attempt to accomplish homologous transformation in the germ line. (e) Characterize structurally and developmentally a homeobox-containing maternal-effect gene, Hb38E, and pair-rule segmentation mutants that map in the same interval. (f) Study the structure and in vivo function of the 205K microtubule-associated protein, the gene for which we have cloned.

我们参与研究真核生物的结构和调节 基因，使用果蝇作为实验对象。 虽然我们来自 各种背景（遗传学，分子生物学，发育生物学， 人群遗传学，细胞生物学），我们以我们的共同利益相交： 使用遗传上最有利的高等生物，果蝇和 当前的分子和细胞生物学技术，以阐明 较高生物体的基因是组织，进化的，并且在 发育和细胞周期。 确实，我们小组的优势是 它代表了互补而又重叠的方法。 在计划项目的背景下，我们打算： （a）通过分子和遗传分析指调的机制 在卵子发生过程中，有序生产绒毛膜蛋白。 （b）通过分子和遗传来研究控制的过程 转转，位置效应和体染色体配对。 （c）通过测序酒精脱氢酶和 源自天然的等源性线的黄嘌呤脱氢酶基因 果蝇pseudoobscura的种群。 （d）表征果蝇的RNA聚合酶II突变体，并尝试 完成种系中的同源转换。 （e）在结构和发展上表征含同源的含量 产妇效应基因，HB38E和配对规则分割突变体，这些突变体在 相同的间隔。 （f）研究205K的结构和体内功能 微管相关蛋白，我们已克隆的基因。