MOLECULAR AND DEVELOPMENTAL GENETICS OF DROSOPHILA

果蝇的分子和发育遗传学

• 批准号: 3096109
• 负责人: FOTIS C KAFATOS
• 金额: $ 95.36万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3096109
• 关键词: biomedical facility; developmental genetics; molecular genetics

We are involved in studying the structure and regulation of eukaryotic genes, using Drosophila as an experimental object. Although we come from diverse background (genetics, molecular biology, developmental biology, population genetics, cell biology), we intersect in our common interest: to use the genetically most favorable higher organism, Drosophila, and the current techniques of molecular and cellular biology, to illuminate how the genes of higher are organized, evolve, and are regulated during development and during the cell cycle. Indeed, a strength of our group is that it represents complementary and yet overlapping approaches. In the context of the program project, we intend to: (a) Test by genetics the in vivo function of transcription factors putatively involved in the developmental regulation of chorion genes, and isolate additional components involved in chorion regulation using enhancer trap method. (b) Study and manipulate the hobo mobile elements, including characterization of the transposase transcript and the mechanism of transposition of the element in the germ line; develop novel transformation vector and procedures. (c) Characterize DNA polymorphism and evolution of the decapentaplegic gene in populations of D. melanogaster and D. pseudoobscura, to obtain the first data on selective constraints on amino acid sequences, message structure and cis-regulatory elements for a gene controlling basic morphogenetic processes. (d) Use genetic approaches to understanding the control of embryonic muscle cell commitment and differentiation, focusing on the nau gene that encodes basic and helix-loop-lelix domains similar to those of vertebrate myogenic regulatory factors. (e) Study the in vitro properties and in vivo function of the products of two genes, ned and nod, which belong to the kinesin superfamily and movements of microtubules in the meiotic and mitotic spindle apparatus.

我们参与研究真核生物的结构和调节 基因，使用果蝇作为实验对象。 虽然我们来自 多种背景（遗传学，分子生物学，发育生物学， 人群遗传学，细胞生物学），我们以我们的共同利益相交： 使用遗传上最有利的高等生物，果蝇和 当前的分子和细胞生物学技术，以阐明 较高的基因是组织，进化的，并在发育过程中受到调节 在细胞周期中。 确实，我们小组的优势是 代表互补而又重叠的方法。 在 计划项目，我们打算： （a）通过遗传学测试转录因子的体内功能 推理地参与了绒毛膜基因的发展调节， 使用增强剂隔离合唱调节涉及的其他组件 陷阱方法。 （b）研究和操纵流浪汉移动元素，包括 转座酶转录本的表征和机制 元素在细菌线中的换位；发展新颖的转型 向量和程序。 （C）表征DNA多态性和脱皮性基因的演变 在D. melanogaster和D. pseudoobscura的种群中，获得第一个 有关氨基酸序列的选择性约束的数据，消息结构 以及控制基本形态发生的基因的顺式调节元素 过程。 （d）使用遗传方法来理解胚胎肌肉的控制 细胞承诺和分化，重点是编码的NAU基因 基本和螺旋 - 环状烯基体域类似于脊椎动物肌原的结构域 监管因素。 （e）研究产物的体外特性和体内功能 NED和点头的两个基因，它们属于运动蛋白超家族， 微管在减数分裂和有丝分裂纺锤体中的运动。