DYNAMICS OF POSTISCHEMIC CALCIUM ACCUMULATION AND PROTEIN SYNTHESIS IN BRAIN

脑缺血后钙积累和蛋白质合成的动力学

• 批准号: 3881854
• 负责人: G MIES
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3881854
• 关键词: brain metabolism; calcium; cerebral ischemia /hypoxia; gamma aminobutyrate; hippocampus; inferior colliculus; laboratory rat; neural inhibition; protein biosynthesis; thalamic nuclei

After 10 minute global of ischemia in rat brain, a marked increase in abnormal calcium uptake and suppression of protein synthesis in nuclei reticularis of thalamus was observed as early as 6 hours postischemia, followed by a significant increase in abnormal accumulation of calcium at moderately reduced rates of protein synthesis in the ventral thalamic nucleus and inferior colliculus which lasted up to 7 days postischemia. In hippocampal CAl sector, protein synthesis of CAl neurons was severely suppressed until 4 days postischemia, but recovered significantly to subnormal levels of protein synthesis after 7 days postischemia. Metabolic amelioration of hippocampal CAl neurons correlated with their improvement of morphological appearance. Our findings suggest l) that early postischemic calcium accumulation occurring predominantly in GABAergic brain areas appears to reflect the consequences of inhibition failure of GABAergic interneurons which may result in a subsequent 'disinhibition' of interconnected projection areas and the development of remote neuron injury; and 2) that following a phase of chronic neuronal injury, hippocampal CAl neurons are able to recover from an ischemic insult.

大鼠大脑中缺血全球10分钟后，明显增加 钙摄取异常和抑制核中蛋白质合成 丘脑的网状会在缺血后6小时观察到 其次是钙的异常积累显着增加 腹丘脑中蛋白质合成的速率适度降低 核和下丘的核切除术后持续7天。在 海马CAL扇形，CAL神经元的蛋白质合成严重 被抑制直到8天后，但已大幅度恢复到 蛋白质合成后7天后的蛋白质合成水平。代谢 海马CAL神经元的改善与其改善相关 形态学外观。我们的发现表明l）早早 在Gabaergic中主要发生的缺血后钙积累 大脑区域似乎反映了抑制失败的后果 GABA能中间神经元可能导致随后的“抑制” 互连的投影区域和远程神经元的发展 受伤; 2）在慢性神经元损伤阶段之后， 海马CAL神经元能够从缺血性侮辱中恢复。