BACTERIAL INTERRELATIONSHIPS IN AN ANIMAL MODEL

动物模型中的细菌相互关系

• 批准号: 3963912
• 负责人: STEPHEN J CHALLACOMBE
• 金额: --
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3963912
• 关键词: Actinomyces; Lactobacillus casei; Streptococcus mutans; bacteria; dental caries; disease /disorder model; enzyme linked immunosorbent assay; germ free condition; oral bacteria; physical chemical interaction

The rodent has been used for a number of years as a model system for the production of human dental caries. (1) the rat has provided to be a suitable model since caries can be readily induced, and the results are in general reproducible (1,2,3). Valuable insights into the cariogenicity of individual bacterial species has been obtained (4,5). More recently the role of host immune factors in the modulation and control of dental caries has been extensively studied in the rat and in the hamster (2,3) and have revealed that either oral or submucosal immunization may protect against caries (2,3,4). In spite of the wealth of data in this field, little is known about the bacterial interrelationships in relation to bacterial colonization and dental caries. In the in vitro model of caries important differences in the production of caries have been found when more than one species of bacteria is used in the model (Heilman and Clarkson). It has been shown that when S. mutans and A. viscossus are fermented with sucrose in the presence of teeth, subsurface lesions in enamel developed with S. mutans and much smaller or no lesions developed with A. viscossus. As no pH differences were detected (both were at pH 4.5) and the teeth were suspended in the media for the same length of time, these results were surprising. However, in the same system both bacteria produced comparable lesions on the root surface. Further investigations are planned to: mix the bacterial strains; to monoinnoculate with S. mutans and then innoculate with the A. Viscossus (to reverse this); to vary the fermation conditions; and to introduce immunological and nonimmunological factors into the system. We wish to extend the in vitro work into a suitable model in vivo, and to be able to study the effects of saliva and host immunological factors on the colonization of bacteria, and on the subsequent development of caries. The rat was selected since this is currently the most well studied animal model and has been shown to be reproducible. For the study of individual bacterial species it is necessary to use a gntobiotic unit.

啮齿动物已被用作模型系统多年 人类龋齿的生产。 （1）老鼠已经提供了 合适的模型，因为可以很容易地诱发龋齿，结果在 一般可重现（1,2,3）。 宝贵的洞察力对商业性的洞察力 已经获得了单个细菌种类（4,5）。 最近 宿主免疫因素在龋齿的调节和控制中的作用 在大鼠和仓鼠（2,3）中已广泛研究 揭示口腔或粘膜免疫可以预防 龋齿（2,3,4）。 尽管该领域的数据丰富，但几乎没有 有关细菌相互关系的了解 殖民和龋齿。 在龋齿的体外模型中 当多个以上 模型（Heilman和Clarkson）中使用了细菌。 它有 已显示出，当S. utans和A. viscossus用蔗糖发酵时 在存在牙齿的情况下，用S.开发的牙釉质中的地下病变。 烟曲霉的变形物和小得多或没有病变。 否 检测到pH差异（均为pH 4.5），牙齿为 这些结果暂停了相同的时间，这些结果是 奇怪。 但是，在同一系统中，两种细菌均可可比 根表面的病变。 计划进一步调查：混合 细菌菌株；用单链链球链球菌进行单渠道，然后无辜 使用A. viscossus（扭转这一点）；改变效费条件； 并将免疫学和非免疫学因素引入 系统。 我们希望将体外工作扩展到合适的体内模型， 并能够研究唾液和宿主免疫学的影响 细菌定殖的因素以及随后的发展 龋齿。 选择老鼠，因为这是目前最井井有条的 研究的动物模型已被证明可再现。 为了研究 在个别细菌物种中，必须使用gntobiotic单位。