EVALUATION OF FREE THYROXIN TECHNIQUES

游离甲状腺素技术的评估

• 批准号: 3837986
• 负责人: M ZWEIG
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3837986
• 关键词: albumins; clinical chemistry; diagnosis quality /standard; radioimmunoassay; radiotracer; serum; thyroid hormone binding protein; thyroxine

Recently, nonisotopic (often automated) immunoassays for measuring serum free thyroxin (FT4) have become available. Though more costly then radioimmunoassays, they are considerably more convenient. We studied the influence of endogenous albumin and thyroxin-binding globulin concentration on five automated, nonisotopic methods of measuring FT4 (Enzymun on ES300 [one-step], Stratus I and II [one-and-a-half-step], Delfia [two-step], and IMx [two-step]). We observed that they (a) are influenced very little by endogenous serum binding proteins and (b) seem to have sufficient within-run precision to justify performing single measurements on patient specimens. Additional studies are now in progress to assess the diagnostic performance of FT4 methods based on the use of radiolabeled T4 or T4 analogs in patients with non-thyroidal illnesses.

最近，用于测量血清的非异位（通常是自动化的）免疫测定 自由甲状腺素（FT4）已可用。 虽然更昂贵 放射免疫分子，它们更方便。 我们研究了 内源白蛋白和甲状腺素结合球蛋白的影响 浓缩五种自动化的非分子方法测量FT4 （eNzymun on Es300 [一步]，II和II [一个半步骤]， Delfia [两步]和IMX [两步]）。 我们观察到他们（a）是 受内源性血清结合蛋白的影响很小，（b）似乎 要具有足够的内部精度以证明执行单身的合理性 对患者标本的测量。 现在正在进行其他研究 根据使用的使用来评估FT4方法的诊断性能 非甲状腺疾病患者的放射性标记的T4或T4类似物。