EVALUATION OF FREE THYROXIN TECHNIQUES

游离甲状腺素技术的评估

• 批准号: 3853109
• 负责人: M ZWEIG
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3853109
• 关键词: albumins; clinical chemistry; hormone binding protein; immunologic techniques; radioimmunoassay; thyroid hormone binding protein; thyroxine

Recently, nonisotopic (often automated) immunoassays for measuring serum free thyroxin (FT4) have become available. Though more costly then radioimmunoassays, they are considerably more convenient. We studied the influence of endogenous albumin and thyroxin-binding globulin concentration on five automated, nonisotopic methods of measuring FT4 (Enzymun on ES300 [one-step], Stratus I and II [one-and-a-half-step], Delfia [two-step], and IMx [two-step]). We observed that they (a) are influenced very little by endogenous serum binding proteins and (b) seem to have sufficient within- run precision to justify performing single measurements on patient specimens.

最近，用于测量血清的非同位素（通常是自动化）免疫分析 游离甲状腺素（FT4）已经可用。 虽然那时候成本比较高 放射免疫测定法，它们要方便得多。 我们研究了 内源性白蛋白和甲状腺素结合球蛋白浓度的影响 测量 FT4 的五种自动化非同位素方法（ES300 上的 Enzymun） [一步]、Stratus I 和 II [一步半]、Delfia [两步] 和 IMx [两步]）。 我们观察到它们 (a) 受以下因素的影响很小 内源性血清结合蛋白和（b）似乎有足够的内- 运行精度以证明对患者进行单次测量的合理性 标本。