GLYCOprotein N-glycosylation from non-life to eukaryotes: a Doctoral Network to expand the knowledge on a ubiquitous posttranslational modification

从非生命到真核生物的糖蛋白 N-糖基化：扩展普遍存在的翻译后修饰知识的博士网络

• 批准号: EP/Y032527/1
• 负责人: Benjamin Schumann
• 金额: $ 33.22万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FY032527%2F1
• 关键词: GLYCOprotein; glycosylation; non; life; eukaryotes

The GLYCO-N training network aims at training Doctoral Candidates (DCs) to acquire the skills to develop different innovative strategies to 1) understand the diversity and structural complexity of archaeal, microalgal and viral N-glycosylation and 2) harness this knowledge for new solutions in biomedicine and biotechnology. Protein N-glycosylation, or the attachment of oligo- and polysaccharides at specific asparagine residues, is conserved throughout life, and is now observed even in the viral world. In contrast to eukaryotes, whose well-studied N-glycosylation machineries are relatively simple, archaea, microalgae and bacteria utilize a wide variety of monosaccharides to create a wealth of structurally diverse N-glycans, and the same holds true for some recently discovered viruses. Because protein glycosylation occurs far downstream of protein synthesis the complexity and diversity in N-glycan structures are poorly understood in detail. This holds true specifically for Nglycosylation events that are the subject of the GLYCO-N program: those in archaea, microalgae and viruses. Understanding of the how and why of N-glycosylation in archaea, microalgae and viruses will open up many possibilities ranging from drug discovery (antivirals) to biotechnology (glycoprotein and glycoprocessing enzyme engineering for materials and life sciences). The GLYCO-N network brings together a diverse group of glycobiology researchers with world-leading expertise in microbiology, (bio)organic chemistry, computational and structural biology, bioinformatics and chemical biology. The GLYCO-N DCs will have their own individual project with one GLYCO-N expert and will, through research internships, be exposed to complementary Glycoscience. All individual PhD projects, while rooted in fundamental science, have a practical application, either in biotechnology or in biomedicine, as will be explored through secondments with our associated partners

GLYCO-N 培训网络旨在培训博士生 (DC)，使其获得开发不同创新策略的技能，以 1) 了解古菌、微藻和病毒 N-糖基化的多样性和结构复杂性，2) 利用这些知识寻找新的解决方案在生物医学和生物技术领域。蛋白质 N-糖基化，即寡糖和多糖在特定天冬酰胺残基上的附着，在整个生命过程中都是保守的，现在甚至在病毒世界中也能观察到。真核生物经过充分研究的 N-糖基化机制相对简单，与之相反，古细菌、微藻和细菌利用多种单糖来产生大量结构多样的 N-聚糖，对于一些最近发现的病毒也是如此。由于蛋白质糖基化发生在蛋白质合成的下游，因此人们对 N-聚糖结构的复杂性和多样性了解甚少。这尤其适用于作为 GLYCO-N 计划主题的 N 糖基化事件：古细菌、微藻和病毒中的 N 糖基化事件。了解古细菌、微藻和病毒中 N-糖基化的方式和原因，将为从药物发现（抗病毒药物）到生物技术（用于材料和生命科学的糖蛋白和糖加工酶工程）开辟许多可能性。 GLYCO-N 网络汇集了一群多元化的糖生物学研究人员，他们在微生物学、（生物）有机化学、计算和结构生物学、生物信息学和化学生物学方面拥有世界领先的专业知识。 GLYCO-N DC 将与一名 GLYCO-N 专家一起开展自己的个人项目，并将通过研究实习接触补充的糖科学。所有单独的博士项目虽然植根于基础科学，但在生物技术或生物医学方面都有实际应用，这将通过与我们的相关合作伙伴借调来探索