Genetic and Functional Dissection of Congenital Anomalies of the Brain
大脑先天性异常的遗传和功能解剖
基本信息
- 批准号:9895872
- 负责人:
- 金额:$ 19.82万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-04-01 至 2021-09-30
- 项目状态:已结题
- 来源:
- 关键词:AblationAffectAnatomyAnencephalyAnimal ModelAntisense OligonucleotidesAwardBioinformaticsBiological AssayBiological ModelsBirthBrainCRISPR/Cas technologyCandidate Disease GeneCell physiologyClinicalClinical ResearchComplementComplexCongenital AbnormalityCongenital cerebellar hypoplasiaCongenital cerebral herniaCongenital neurologic anomaliesCounselingDataDefectDevelopmentDiagnosisDiagnosticDideoxy Chain Termination DNA SequencingDiseaseDisease modelDissectionEmbryoEnrollmentEtiologyFamilyFetusFundingFutureGenesGeneticGenetic studyGenomeGenomicsGenotypeHealthcare SystemsHeterogeneityHoloprosencephalyHumanHybridsImageInvestigationKaryotypeKnowledgeLarvaLeadLesionLightLiteratureMagnetic Resonance ImagingMessenger RNAMicrocephalyModelingMolecularMolecular DiagnosisMutateNervous system structureNeuraxisNeurological observationsNewborn InfantOpticsOrganOrthologous GeneParentsPathogenicityPhenotypePhysiciansPhysiologicalPopulation GeneticsPositioning AttributePregnancyPrenatal DiagnosisPreventiveProcessProteinsRecurrenceResolutionRiskSchizencephaly ScientistSignal PathwaySingle Nucleotide PolymorphismStructureSyndromeSystemTechnologyTestingTherapeuticTranslatingTranslational ResearchUltrasonographyUnited StatesVariantWorkZebrafishbasebody systembrain abnormalitiesbrain malformationcausal variantclinical Diagnosisclinical careclinical phenotypeexomeexome sequencingexperiencefetalgene discoverygenetic analysisgenetic disorder diagnosisgenetic pedigreegenetic variantgenome editinggenome-widegenomic datahuman diseasehuman modelimprovedin vivoin vivo Modelinnovationinsertion/deletion mutationknock-downlissencephalymultidisciplinarynervous system developmentnoveloutcome forecastperinatal periodphenotypic dataprenatalprospectiverare varianttherapeutic targettoolzebrafish genome
项目摘要
PROJECT SUMMARY
Human brain development remains an incompletely understood process, yet congenital abnormalities of this
complex structure affect approximately 3/1,000 pregnancies and more than 2000 newborns annually in the
United States, posing a substantial burden on the health care system. Congenital brain abnormalities,
hallmarked by vast phenotypic heterogeneity, include but are not limited to holoprosencephaly,
schizencephaly, anencephaly, encephalocele, microcephaly, ventriculomegaly, cerebellar hypoplasia, and
disorders of cortical development, such as lissencephaly. The paired approach of: (1) prenatal diagnosis using
a combination of ultrasound and fetal MRI to characterize aberrant phenotypes; with (2) genetic analysis to
determine causal lesions, has greatly improved the ability to accurately counsel families about diagnosis,
prognosis, and recurrence risk. More recently, prenatal whole exome sequencing (WES) has been applied in
cases of lethal or multiple fetal abnormalities to make a molecular diagnosis that otherwise could not be
identified with traditional testing. Pilot data from our group and others using WES show a diagnostic rate of 16-
30% in cases of multiple fetal abnormalities, but only 1-2% in isolated brain abnormalities, indicating a critical
need to improve diagnostic capabilities and identify novel genes critical to human brain development. We posit
that the overabundance of unresolved fetal cases is in large part due to: (1) a knowledge gap in our
understanding of the repertoire of genotypes underlying brain abnormalities with prenatal onset; and (2)
limitations of population genetics to establish causality of rare variants in novel candidate genes. Here, two
CTSA-funded teams who are at the forefronts of prenatal genetic diagnostics and in vivo zebrafish modeling of
human disease, at UNC and Duke, respectively, will team up to overcome the current challenges of diagnosing
brain abnormalities with a prenatal onset. We will intersect exome- and genome-wide variation data with
experimentally tractable and relevant model systems, zebrafish (Danio rerio). We hypothesize that
bioinformatics filters using prenatal WES data will reveal novel candidate genes, which can be applied to a
zebrafish model to generate initial discoveries critical to human brain development and translate into improved
clinical care. First, we will perform bioinformatic analysis of 10 clinically ascertained fetuses with CNS
anomalies and their parents using a tiered filtering strategy; and we will apply this analysis paradigm iteratively
to 32 prospectively enrolled fetuses and their families. Second, we will establish relevance of candidate genes
to brain development and determine variant pathogenicity using state-of-the-art genome editing and
phenotyping tools in zebrafish. Completion of our work will expand our understanding of the molecular
processes governing prenatal brain development; establish a clinical-research hybrid platform readily
applicable to other anatomical organ defects detectable by fetal imaging; and build a suite of animal models of
aberrant CNS development with potential for future use in therapeutic target identification.
项目摘要
人脑发育仍然是一个不完全理解的过程,但先天性异常
复杂的结构每年在
美国,对医疗保健系统造成了重大负担。先天性大脑异常,
以广阔的表型异质性为标志,包括但不限于全脑脑,
精神分裂,Ancephaly,脑核,小头畸形,心室肿瘤,小脑呼吸症和
皮质发育的疾病,例如Lissencephaly。配对方法的:(1)使用产前诊断
超声和胎儿MRI的组合以表征异常表型; (2)遗传分析
确定因果病变,已大大提高了准确咨询家庭诊断的能力,
预后和复发风险。最近,在
致命或多个胎儿异常的病例,以做出分子诊断,否则不可能是
通过传统测试确定。来自我们小组和其他使用WE的其他人的飞行员数据显示16--的诊断率为16-
在多种胎儿异常的情况下,有30%,但孤立的脑异常中只有1-2%,表明关键
需要提高诊断能力并确定对人脑发育至关重要的新基因。我们认为
未解决的胎儿病例的过度在很大程度上是由于:(1)我们的知识差距
了解产前发作的脑异常基因型的曲目; (2)
种群遗传学在新候选基因中建立稀有变异的因果关系的局限性。在这里,两个
CTSA资助的团队处于产前遗传诊断和体内斑马鱼建模的最前沿
分别在UNC和Duke的人类疾病将联手克服当前诊断的挑战
发作产前的脑异常。我们将与全基因组和全基因组变异数据相交
实验可牵引和相关的模型系统,斑马鱼(Danio Rerio)。我们假设这一点
使用产前WES数据的生物信息学过滤器将揭示新的候选基因,可以应用于A
斑马鱼模型生成对人脑发育至关重要的初始发现并转化为改进的
临床护理。首先,我们将对CNS的10个临床确定的胎儿进行生物信息学分析
异常及其父母使用分层的过滤策略;我们将在迭代上应用此分析范式
到32名前瞻性招募的胎儿及其家人。其次,我们将建立候选基因的相关性
使用最先进的基因组编辑和
斑马鱼中的表型工具。完成工作将扩大我们对分子的理解
处理产前大脑发育的过程;轻松建立一个临床研究混合动力平台
适用于可通过胎儿成像检测到的其他解剖器官缺陷;并建立一套动物模型
异常的CNS开发具有未来在治疗靶标识别中使用的潜力。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Erica Ellen Davis其他文献
Erica Ellen Davis的其他文献
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{{ truncateString('Erica Ellen Davis', 18)}}的其他基金
Functional dissection of GnRH defects and networks
GnRH 缺陷和网络的功能剖析
- 批准号:
9910434 - 财政年份:2020
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Functional Dissection of CNVs in Neurodevelopmental Traits
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10107962 - 财政年份:2020
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Genetic and Functional Studies of Human Ciliary Syndromes
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10188509 - 财政年份:2019
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$ 19.82万 - 项目类别:
Genetic and Functional Dissection of Congenital Anomalies of the Brain
大脑先天性异常的遗传和功能解剖
- 批准号:
9752755 - 财政年份:2019
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$ 19.82万 - 项目类别:
Functional Dissection of CNVs in Neurodevelopmental Traits
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10366987 - 财政年份:2015
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10491188 - 财政年份:2015
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