REAGENT PRODUCTION SUPPORT FOR RESEARCH ON AIDS

艾滋病研究的试剂生产支持

• 批准号: 2295826
• 负责人: MARIAN GORECKI
• 金额: $ 0.02万
• 依托单位: BIO-TECHNOLOGY GENERAL CORPORATION
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-10 至 1996-06-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2295826
• 关键词: REAGENT; PRODUCTION; SUPPORT; RESEARCH; AIDS

The purpose of this contract is to support the National Institute of Allergy and Infectious Diseases (NIAID) in its mission to stimulate research towards discovery of improved therapy for the acquired immunodeficiency syndrome (AIDS) and associated opportunistic infections. The NIAID requires production of state-of-the-art reagents to support laboratory research efforts of AIDS investigators. The development of antiviral and anti-infective drugs and vaccines effective against HIV and associated opportunistic microorganisms has been hampered by the absence of standardized, freely available reagents. Such reagents are necessary for the production of specific drug targets in vitro so that efficient screening of candidate synthetic compounds can proceed expeditiously. Because of technical difficulties in the propagation of opportunistic pathogens in vitro, molecular reagents are urgently needed to develop improved methods to monitor organism load in patients undergoing treatment. The contractor will receive reagents and starting materials from the Project Officer for the purpose of modifying, amplifying, producing, and shipping molecular biology reagents to the NIAID AIDS Research and Reference Reagent Program for distribution to NIAID-supported investigators. It is the objective of this activity to prepare, evaluate, and amplify cDNA and genomic libraries; prepare expression vectors and produce gene products; to prepare, produce, and purify soluble proteins; to prepare and produce polyclonal and monoclonal antibodies; and to assay, evaluate, describe, and ship the resulting reagents to the NIAID and to scientific investigators studying opportunistic infections. The products produced under this contract will be used to support the research activities of the scientific community. The NIAID needs access to significant quantities of the noted materials to support the efforts of the National Cooperative Drug Discovery Groups, the AIDS Clinical Study Groups, the Programs of Excellence in Basic Research on AIDS, and other specific programs initiated by NIAID.

本合同的目的是支持国家研究所 过敏和传染病 (NIAID) 的使命是刺激 研究发现改进的治疗方法 免疫缺陷综合症（艾滋病）和相关的机会性感染。 NIAID 需要生产最先进的试剂来支持 艾滋病调查人员的实验室研究工作。 的发展 有效对抗艾滋病毒和艾滋病毒的抗病毒和抗感染药物和疫苗 相关的机会微生物因缺乏而受到阻碍 标准化的、免费提供的试剂。 此类试剂是必需的 用于体外生产特定药物靶标，从而高效 候选合成化合物的筛选可以迅速进行。 由于传播机会主义的技术困难 病原体体外检测，分子试剂亟待研发 改进的方法来监测接受治疗的患者的有机负荷 治疗。 承包商将从 项目官员负责修改、放大、制作和 向 NIAID 艾滋病研究中心运送分子生物学试剂 用于分发给 NIAID 支持的参考试剂计划 调查人员。 这项活动的目的是准备、评估、 并扩增 cDNA 和基因组文库；准备表达载体和 产生基因产物；制备、生产和纯化可溶性蛋白质； 制备和生产多克隆和单克隆抗体；并进行测定， 评估、描述所得试剂并将其运送至 NIAID 并 研究机会性感染的科学研究人员。 产品 根据本合同生产的产品将用于支持研究 科学界的活动。 NIAID 需要访问 大量的上述材料来支持 国家药物发现合作小组、艾滋病临床研究 艾滋病基础研究卓越计划和其他 NIAID 发起的具体计划。