Broadening access with an Armamentarium Vector Core Powered by Inclusive Research Experiences - SUPPLEMENT

通过包容性研究经验支持的 Armamentarium 矢量核心扩大访问范围 - 补充

基本信息

批准号：
10885509
负责人：
Viviana Gradinaru
金额：
$ 15.57万
依托单位：
CALIFORNIA INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-01 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10885509
关键词：
Adopted African American student Award BRAIN initiative Biological Biological Assay Biotechnology Brain California Capsid Cataloging Cell Culture Techniques Collaborations Communities Custom Dedications Dependovirus Disease Diverse Workforce Dose Ecosystem Education Educational workshop Electronics Eligibility Determination Engineering Ensure Equipment and supply inventories Equity Feedback Fees Fostering Freezing Funding Gene Delivery Generations Genome Hispanic-serving Institution Human Immunohistochemistry Infrastructure Injections Link Mammalian Cell Mentors Methodology Methods Minority-Serving Institution Mission Modernization Molecular Monitor Mus Neuromodulator Neurons Neurosciences Population Process Production Publications Quality Control Reagent Research Research Personnel Resources Running Science Scientist Site Structure Students Support System System Training Tropism Underrepresented Minority United States National Institutes of Health Universities Validation Virus Work adeno-associated viral vector aluminum sulfate career cell type experience gene therapy innovation instrumentation meetings next generation novel parent grant posters pre-doctoral programs quality assurance science and society sensor sex summer program tool undergraduate research undergraduate student vector virus identification web site

项目摘要

PROJECT SUMMARY (Changes from awarded parent grant in bold) The AAV BRAIN Safe and Effective Neuromodulator and Sensor Utilization across Species (SENSUS, led by Caltech) of the NIH BRAIN Initiative Armamentarium Project will develop, validate, and disseminate integrated (capsid and genome) engineered adeno-associated virus (AAV) tools to monitor and manipulate molecularly defined neuronal cell types across vertebrate species. To broaden access to these reagents within the neuroscientific community, we propose a close partnership between the AAV BRAIN SENSUS and California State Polytechnic University, Pomona (CPP), which will host a new Armamentarium Vector Core (ArmVC). This collaboration will also increase access to the research community itself for underrepresented minorities (URM) and minority-serving institutions (MSI), as CPP is a designated Hispanic-Serving Institution that also serves large populations of Pell-eligible, first-generation, and African American students. This partnership will foster close interfacing between experts in AAV engineering for neuroscience (PI Gradinaru), AAV production innovations and dissemination (PI Shay), and URM undergraduate research and mentoring (Co-I Steele) to build the physical and human infrastructure for AAV production, validation, and dissemination at CPP while comprehensively integrating MSI undergraduates. Caltech’s CLOVER Center (directed by PI Shay) will utilize their experience in running a successful AAV dissemination center to work with Co-I Steele and Damien Wolfe, M.S., a CPP and Steele lab alum and CLOVER technician who will work at ArmVC, to establish robust AAV production and dissemination capabilities in the ArmVC at CPP (Aim 1). CLOVER and ArmVC will cooperatively run a new AAV BRAIN SENSUS Program for Inclusive Research Experience (ASPIRE) wherein CPP undergraduate students chosen as ASPIRE Scientists will produce validated AAV for dissemination to the neuroscience community as part of the new ArmVC (Aim 2) and work in Co-I Steele’s lab to independently validate, using immunohistochemistry, the novel AAV identified via high-throughput methods in PI Gradinaru’s lab (Aim 3). ASPIRE Scientists will benefit from activities including workshops, poster presentations, research seminars, and career-focused mentoring meetings with PI Gradinaru at CPP and Caltech. Yearly training (and re-training of returning staff and undergraduates) in AAV production by CLOVER staff will ensure that methodological advances in AAV production, purification, and characterization developed or adopted by the CLOVER center are quickly transferred between sites. ArmVC offerings will be publicized and disseminated through a stand-alone website while also being integrated into AAV BRAIN SENSUS electronic resources for seamless reagent cataloguing and feedback. With strong quality control and quality assurance processes in place and overseen by CLOVER, the ArmVC at CPP will broaden access to modern neuroscientific tools and help bridge the gap in URM career progression in research science.

项目摘要（粗体字为授予家长补助金的变化） AAV BRAIN 跨物种安全有效的神经调节剂和传感器利用（SENSUS，由 NIH BRAIN Initiative Armamentarium 项目的加州理工学院 (Caltech) 将开发、验证和传播集成的（衣壳和基因组）工程腺相关病毒（AAV）工具用于分子监测和操作确定跨脊椎动物物种的神经细胞类型，以扩大对这些试剂的使用范围。在神经科学界，我们建议 AAV BRAIN SENSUS 与加州建立密切的合作伙伴关系波莫纳州立理工大学 (CPP) 将举办新的 Armamentarium Vector Core (ArmVC)。合作还将增加代表性不足的少数群体（URM）进入研究界本身的机会和少数族裔服务机构 (MSI)，因为 CPP 是指定的拉美裔服务机构，也为大型机构提供服务这种伙伴关系将促进符合佩尔资格的第一代学生和非裔美国学生的紧密联系。神经科学 AAV 工程 (PI Gradinaru)、AAV 生产创新领域专家之间的交流和传播（PI Shay），以及 URM 本科生研究和指导（Co-I Steele）来建立物理 CPP 的 AAV 生产、验证和传播的人力基础设施，同时全面整合 MSI 本科生。加州理工学院的 CLOVER 中心（由 PI Shay 领导）将利用他们成功运行 AAV 的经验传播中心与 Co-I Steele 和 Damien Wolfe（硕士）、CPP 和 Steele 实验室校友以及 CLOVER 合作技术人员将在 ArmVC 工作，以建立强大的 AAV 生产和传播能力 CPP 的 ArmVC（目标 1）将与 ArmVC 合作运行一个新的 AAV BRAIN SENSUS 计划。包容性研究经验 (ASPIRE)，CPP 本科生被选为 ASPIRE 科学家将生产经过验证的 AAV，作为新 ArmVC 的一部分传播到神经科学界（目标 2）并在 Co-I Steele 的实验室工作，利用免疫组织化学技术独立验证了发现的新型 AAV 通过 PI Gradinaru 实验室的高通量方法（目标 3），ASPIRE 科学家将受益于包括以下活动在内的活动。研讨会、海报展示、研究研讨会以及 PI Gradinaru 的职业指导会议在 CPP 和加州理工学院。 CLOVER 每年进行 AAV 生产培训（以及回国员工和本科生的再培训）工作人员将确保 AAV 生产、纯化和表征方面的方法学进步或由 CLOVER 中心通过的 ArmVC 产品将在站点之间快速转移。通过独立网站传播，同时也集成到 AAV BRAIN SENSUS 电子版中无缝试剂编目和反馈的资源具有强大的质量控制和质量保证。 CPP 的 ArmVC 已到位并由 CLOVER 监督，将拓宽现代神经科学的获取途径工具并帮助缩小研究科学领域 URM 职业发展的差距。

项目成果

期刊论文数量（1）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors.

DOI：
10.1126/sciadv.adg6618
发表时间：
2023-04-21
期刊：
SCIENCE ADVANCES
影响因子：
13.6
作者：
Shay, Timothy F.;Sullivan, Erin E.;Ding, Xiaozhe;Chen, Xinhong;Kumar, Sripriya Ravindra;Goertsen, David;Brown, David;Crosby, Anaya;Vielmetter, Jost;Wolfe, Damien A.;Lam, Annie W.;Gradinaru, Viviana
通讯作者：
Gradinaru, Viviana