MECHANISM OF CELLULAR ADHESION

细胞粘附机制

• 批准号: 3756560
• 负责人: STEVEN B OPPENHEIMER
• 金额: --
• 依托单位: CALIFORNIA STATE UNIVERSITY NORTHRIDGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3756560
• 关键词: alternatives to animals in research; carbohydrate receptor; cell adhesion; cell cell interaction; gel electrophoresis; histogenesis; immunofluorescence technique; invertebrate embryology; laboratory rabbit; minority institution research support; sea urchins; tissue /cell culture

The development of embryos and the spread of cancer are dependent upon adhesive interactions of cells. This laboratory has been engaged in cell adhesion studies for two decades. Thirty of our publications in refereed journals have been co-authored by our undergraduate and pre-masters students (81 student co-author citations). The lab has been continuously funded from a variety of research and science education sources (totaling about $2 million) and therefore this request is for Associate status. Presently 25 students are engaged in research in this laboratory, many of whom are underrepresented, including 2 MARC and 4 MAERC Fellows, who already have made 9 presentations at professional meetings in the past year. 36 students from this lab are now Ph.D's, in Ph.D. programs and/or are involved in biomedical science. Our ongoing projects investigate the mechanisms of cell-cell adhesion in the sea urchin embryo, a model for such studies because of material availability, complete transparency of the embryos and simplicity of the medium (sea water). The MBRS students, just like our MARC and MAERC students, will be fully involved in experimental design, execution, preparation for publication and presentation of the research. Using western cell adhesion and polyacrylamide gel electrophoresis, the students will characterize cell adhesion molecules isolated from living sea urchin blastula cells that promote cell adhesion in a species-specific and developmental stage specific manner. Using beads derivatized with specific sugars and lectins, reduced (non-agglutinating) lectins and hapten sugars, they will study the role of specific sugars and sugar receptors in the adhesion mechanism. They will also become engaged in exciting new experiments, that involve entering the intact, living embryo with sugar, enzyme and reduced lectin reagents that will help us determine if specific sugars are involved in adhesive interactions that are pivotal in morphogenesis. These will be among the first experiments anywhere that probe physiologically important in vivo adhesion mechanisms in this model system.

胚胎的发育和癌症的扩散取决于 细胞间的粘附相互作用。本实验室一直从事细胞 粘附力研究已有二十年历史。 我们的 30 篇出版物已获得审稿 期刊由我们的本科生和硕士预科生共同撰写 学生（81 名学生共同作者引用）。实验室一直在不断地 由各种研究和科学教育来源资助（总计 约 200 万美元），因此此请求是针对助理身份的请求。 目前有25名学生在该实验室从事研究工作，其中许多 代表性不足，包括 2 名 MARC 和 4 名 MAERC 研究员， 过去已在专业会议上做过 9 次演讲 年。该实验室现有博士生36人，在读博士生1人。程序和/或 涉及生物医学科学。 我们正在进行的项目研究细胞与细胞粘附的机制 海胆胚胎，因其材料而成为此类研究的模型 可用性、胚胎完全透明以及操作简单 介质（海水）。 MBRS 的学生，就像我们的 MARC 和 MAERC 一样 学生将全面参与实验设计、执行、 为研究的出版和展示做准备。使用 Western细胞粘附和聚丙烯酰胺凝胶电泳，学生 将表征从活海胆中分离出的细胞粘附分子 囊胚细胞可促进物种特异性和细胞粘附 发展阶段的具体方式。使用特定衍生化的珠子 糖和凝集素、还原（非凝集）凝集素和半抗原糖， 他们将研究特定糖和糖受体在 粘附机制。他们还将从事令人兴奋的新活动 实验涉及用糖进入完整的活胚胎， 酶和还原凝集素试剂将帮助我们确定是否具有特异性 糖参与粘附相互作用，这对于 形态发生。这些将是任何地方的首批实验之一 在此模型中探索生理上重要的体内粘附机制 系统。