SPINAL REGULATION OF SOMATIC SENSATIONS

躯体感觉的脊柱调节

基本信息

批准号：
3406012
负责人：
JERRY COLLINS
金额：
$ 12.38万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1986
资助国家：
美国
起止时间：
1986-07-01 至 1989-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3406012
关键词：
afferent nerve barbiturates cats dorsal column dorsal raphe nucleus electrophysiology local anesthetics neural transmission neuropharmacology sensorimotor system serotonin inhibitor single cell analysis stimulus /response

项目摘要

The objective of this research plan is to begin to evaluate the effects of descending supraspinal modulation on spinal dorsal horn (Rexed laminae IV-VI) neurons. This proposal is unique in that it will involve extracellular recordings from single spinal neurons in intact, awake cats. This will permit an evaluationof the systems as they normally function without confounding influences of anesthesia decerebration or spinal cord transection. Descending inhibitory effects on dorsal horn neurons have been demonstrated, including the assumed analgesic-producing effect of descending inhibition of wide dynamic range (WDR) neurons. This assumed mechanism of analgesia is open to question, however, because of the unknown physiologic role of WDR neurons, as well as the ability of descending inhibition to alter non-nociceptive activity as well. Several lines of evidence would suggest that in the intact animal, descending inhibition may block the ability of dorsal horn neurons to respond with a wide dynamic range profile. In spite of the lack of a clear appreciation of the physiologic role of wide dynamic range neurons. They have been the center of many studies which have evaluated the ability of either physiological or pharmacological manipulations to both alter dorsal horn neuronal activity as well as produce behavioral analgesia. Because of their potentially important role in our understanding of the neurophysiology of analygesia. it is essential that we have a clear appreciation of factors that regulate wide dynamic range neuron response properties in the intact animal. As such, this proposal has, as its specific aims, the following: 1) to determine if tonically active descending inhibition blocks the ability of spinal dorsal horn lumbar neurons to respond with a wid dynamic range profile in the physiologically intace, awake, drug free, behaviorally naive cat. 2) to determine if descending inhibition from the nucleus raphe magnus is involved in this modulation of WDR profiles. 3) to determine if descending inhibition from the nucleus raphe magnus also contributes to the depression of spontaneous activity of dorsal horn neurons in intact animals. 4) to evaluate the effect of serotonin blockage on dorsal horn neurons in the intact animal.

该研究计划的目的是开始评估脊柱背喇叭上脊柱上脊髓调制（Rexed Laminae IV-VI）神经元。该提议的独特之处在于它将涉及来自完整，清醒猫的单个脊柱神经元的细胞外记录。这将允许系统正常运行时的评估没有混淆麻醉或脊髓的影响横向。对背角神经元的降低抑制作用具有已被证明，包括假定的镇痛产生效应下降抑制广泛的动态范围（WDR）神经元。这是假设的但是，由于未知 WDR神经元的生理作用以及下降的能力抑制也改变了非伤害性活性。几行有证据表明，在完整的动物中，下降的抑制可能阻止背角神经元以广泛的动态反应的能力范围配置文件。尽管缺乏对广泛动态范围神经元的生理作用。他们一直是中心在评估生理学或两者都会改变背角神经元活动的药理操作以及产生行为镇痛。因为他们的潜在我们对分析神经生理学的理解中的重要作用。至关重要的是，我们对规范的因素有明确的认识完整动物中广泛的动态范围神经元反应特性。作为这样的提案具有以下目的，以下是：1）确定刺激性降抑制是否会阻止脊柱背喇叭腰神经元以宽度动态范围反应在生理上插入，清醒，无药物，行为上天真的轮廓猫。 2）确定是否会从核核中降低抑制作用 Magnus参与了WDR轮廓的调制。 3）确定是否从核Raphe Magnus降低抑制也有助于完整的背角神经元自发活动的抑郁动物。 4）评估5-羟色胺阻塞对背角的影响完整动物中的神经元。