INHALATION ANESTHETIC EFFECTS ON THE SPINAL DORSAL HORN

吸入麻醉对脊髓背角的影响

• 批准号: 2182887
• 负责人: JERRY COLLINS
• 金额: $ 20.87万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-06-01 至 1996-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2182887
• 关键词: action potentials; anesthesia complication; anesthetics; catheterization; cats; computer data analysis; dorsal horn; electrophysiology; enflurane; halothane; inhalation anesthesia; isoflurane; neuropharmacology; nitrous oxide; sensory thresholds; spinal cord mapping

The goal of this research plan is to examine the effects of inhalation anesthetic agents on sensory processing in the spinal dorsal horn of physiologically intact, awake, drug-free animals. The proposal is unique in that it will employ extracellular recordings from neurons in intact, awake animals allowing evaluation of systems as they function normally without confounding influences of anesthesia, decerebration or spinal cord transection. We have recently demonstrated a profound effect of pentobarbital and propofol on spinal dorsal horn neurons but an apparent lack of similar effects by ketamine. An understanding of the mode of action of general anesthetics is an elusive but important goal. By documenting specific effects of anesthetic agents on sensory neurons in the spinal dorsal horn, we hope eventually to be able to define the pharmacology responsible for the observed changes- knowledge that may provide further insights into the mechanisms of action of general anesthetics. While recording extracellular activity from single spinal dorsal horn neurons in physiologically intact, awake, drug-free animals, we propose to determine baseline neuronal responses to noxious and non-noxious somatic stimuli induce anesthesia with the short-acting intravenous anesthetic propofol and maintain anesthesia with varying concentrations of halothane, enflurane, isoflurane and nitrous oxide. At a time when propofol effects have dissipated and an equilibrium state is established with the inhalation agent, we will evaluate the neuronal response to the same stimuli used in baseline studies. These results, when combined with data from intravenously administered anesthetics will help to define the effect of general anesthetics on sensory processing in spinal dorsal horn neurons and may provide insight into the mechanisms of general anesthesia.

该研究计划的目的是检查吸入的影响 麻醉剂在脊柱背角的感觉加工 生理上完整，清醒的无药物。该提议是独一无二的 因为它将使用完整的神经元的细胞外记录， 醒着动物可以在系统正常运行时评估系统 没有混淆麻醉，解剖或脊柱的影响 绳索横切。 我们最近证明了 戊巴比妥和丙泊酚在脊柱背角神经元上，但明显 氯胺酮缺乏类似的作用。 了解 一般麻醉的行动是一个难以捉摸但重要的目标。 经过 记录麻醉剂对感觉神经元的特定影响 脊柱角，我们希望最终能够定义 负责观察到的变化的药理学可能 提供对一般行动机制的进一步见解 麻醉药。 同时记录单个脊髓角的细胞外活性 我们建议的 确定对有害和无毒理的基线神经元反应 体细胞刺激引起麻醉，静脉静脉 麻醉丙泊酚并保持麻醉浓度不同 Halothane，Enflurane，异氟烷和一氧化二氮。 在 丙泊酚效应已经消散，并建立了平衡状态 使用吸入剂，我们将评估神经元反应 基线研究中使用的相同刺激。 这些结果，当合并时 有了静脉内管理麻醉药的数据将有助于 定义全身麻醉对脊柱感觉处理的影响 背喇叭神经元，可能会深入了解 全身麻醉。