INHALATION ANESTHETIC EFFECTS ON THE SPINAL DORSAL HORN

吸入麻醉对脊髓背角的影响

• 批准号: 2022413
• 负责人: JERRY COLLINS
• 金额: $ 18.56万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-06-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2022413
• 关键词: anesthesia complication; anesthetics; cats; dorsal horn; drug screening /evaluation; electrophysiology; enflurane; excitatory aminoacid; halothane; inhalation anesthesia; isoflurane; laboratory rat; microelectrodes; neurons; neuropharmacology; neurotransmitter agonist; neurotransmitter antagonist; neurotransmitter receptor; neurotransmitters; nitrous oxide; sensory thresholds; stimulus /response

A long-term goal of our laboratory is to understand how general anesthetics alter sensory processing in the spinal cord. Recent work in our laboratory has revealed anesthetic related changes in the size of peripheral receptive fields and neuronal responses to non-noxious receptive field stimulation. The specific aim of this research project is to use those observed anesthetic effects as a tool to define what transmitter systems are involved in modulation of spinal sensory processing by selected general anesthetic agents. That information will provide a better understanding of the pharmacologic mechanisms by which general anesthetics alter nervous system function. Two separate protocols will be employed to answer the following 4 questions: 1.) What neurotransmitter systems contribute to the ability of halothane to depress receptive field size and neuronal response to receptive field brushing? 2.) What neurotransmitter systems contribute to enflurane's ability to depress receptive field size while at the same time enhancing neuronal response to brushing of the remaining receptive field? 3.) Does the new inhalation anesthetic desflurane differ from halothane and enflurane in its effects on spinal sensory processing? 4.) What neurotransmitter systems contribute to the ability of desflurane to alter spinal sensory processing? Questions 1, 2, and 4 will be answered by studying the effects of systemically and/or spinally administered neurotransmitter receptor agonists and antagonists on halothane, enflurane and desflurane induced alterations in spinal dorsal horn neuronal responses to non-noxious stimulation of peripheral receptive fields in acute rat preparations. Question 3 will be answered by examining the effects of desflurane on spinal dorsal horn neuronal responses to non-noxious and noxious receptive field stimulation in a chronic cat preparation. These studies should define, at the receptor level, systems that contribute to the ability of selected anesthetics to alter spinal sensory processing. While ultimate mechanisms of action are molecular in nature, results from this study will help to define neurotransmitter systems that are likely to be the mechanism by which molecular actions of anesthetics are translated into systemic effects that alter the ability of the nervous system to process sensory information.

我们实验室的长期目标是了解一般 麻醉药会改变脊髓中的感觉处理。最近的工作 我们的实验室揭示了与麻醉相关的变化 周围接受场和对非毒性的神经元反应 接受场刺激。该研究项目的具体目的是 使用观察到的麻醉效果作为定义什么的工具 发射机系统参与脊柱感觉的调节 通过选定的一般麻醉剂处理。这些信息将 提供对药理机制的更好理解 一般麻醉剂改变神经系统功能。 将使用两个单独的协议来回答以下4 问题：1。）哪些神经递质系统有助于 氟烷以降低接受场的大小和神经元反应 接受场刷牙？ 2.）哪些神经递质系统有助于 Enflurane降低接收场大小的能力同时 增强对剩余接收场刷牙的神经元反应？ 3.）新的吸入麻醉剂与卤代不同 和Enflurane对脊柱感觉加工的影响？ 4.）什么 神经递质系统有助于Desflurane改变 脊柱感觉处理？ 问题1、2和4将通过研究 全身和/或身经旋转神经递质受体 氟烷，enflurane和desflurane诱导的激动剂和拮抗剂 脊柱背角神经元反应对非毒性的改变 刺激急性大鼠制剂中的外周接受场。 问题3将通过检查Desflurane对 脊髓背角神经元反应对非敏感和有害的接受 慢性猫制剂中的现场刺激。 这些研究应在受体水平上定义 有助于选择麻醉剂改变脊柱感觉的能力 加工。尽管作用的最终机制本质上是分子的 这项研究的结果将有助于定义神经递质系统 可能是麻醉分子作用的机制 被翻译成系统性效应，改变了神经的能力 处理感官信息的系统。