ACTIVATION OF GENES IN THE AGING BRAIN AND IN SCRAPIE

衰老大脑和瘙痒症中基因的激活

• 批准号: 3406942
• 负责人: ASHLEY T. HAASE
• 金额: $ 12.39万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3406942
• 关键词: Alzheimer's disease; Downs syndrome; aging; autoradiography; brain; complementary DNA; gel electrophoresis; gene expression; genetic disorder diagnosis; genetic library; genetic manipulation; genetic transcription; hamsters; human old age (65+); human tissue; in situ hybridization; laboratory mouse; laboratory rabbit; messenger RNA; methylation; molecular cloning; nucleic acid hybridization; nucleic acid sequence; pathologic process; prions; scrapie

We have recently constructed a cDNA library from the brain mRNAs of animals infected with the scrapie agent. Using differential screening and in situ hybridization we have found a cDNA clone to a gene whose expression increases in scrapie. This cloned DNA probe binds to cells in the brains of infected animals, to the senile plaques in Alzheimer's dementia (AD), and to cells in the brain tissues from elderly patients. We propose to continue the analysis of this clone and others in the library in order to gain insight into the nature of the scrapie agent and the origins of pathological alterations in the aging brain that occur in scrapie and AD. We also plan a more direct approach in the pathology of AD using the same strategy of differential screening of a library from the mRNAs of AD, to find genes whose ativity is increased in the aging brain and genes which by in situ hybridization have the appropriate tissue and cellular localization to suggestive involvement in the pathological process.

我们最近构建了一个来自大脑的 cDNA 文库 感染痒病剂的动物的 mRNA。 使用 通过差异筛选和原位杂交，我们发现了 cDNA 克隆到在痒病中表达增加的基因。 这 克隆的 DNA 探针与受感染动物大脑中的细胞结合， 阿尔茨海默氏痴呆 (AD) 中的老年斑，以及 来自老年患者的脑组织。 我们建议继续 分析该克隆和文库中的其他克隆以获得 深入了解瘙痒症病原体的性质和起源 瘙痒病中大脑老化的病理改变 和广告。 我们还计划在病理学方面采取更直接的方法 AD 使用相同的文库差异筛选策略 从 AD 的 mRNA 中寻找活性增强的基因 在衰老的大脑和基因中，通过原位杂交 适当的组织和细胞定位以提示 参与病理过程。