ENZYMES AND METABOLITES OF THE PURINE PATHWAY IN RAT LIVER

大鼠肝脏中嘌呤途径的酶和代谢物

• 批准号: 3801932
• 负责人: R L VEECH
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3801932
• 关键词: acetates; alcohol dehydrogenase; cofactor; enzyme inhibitors; enzyme mechanism; enzyme substrate; ethanol; hyperuricemia; laboratory rat; liver metabolism; pentose phosphate shunt; phosphates; purine /pyrimidine metabolism; pyrophosphates

The metabolism of purine in liver is being investigated, particularly in regard to the effects of ethanol administration. In man, alcohol ingestion or infusion causes elevated uric acid production and hyperuricemia. These changes are accompanied by elevated acetate and phosphate concentrations in the blood, and elevated acetate, phosphate and pyrophosphate contents in rat liver. We are investigating in rats, the effect of ethanol administration, given in the absence or presence of an inhibitor of alcohol dehydrogenase; the effects of the metabolic product acetate; and the effects of phosphate, the plasma concentration, of which is known to fluctuate with ethanol ingestion. These compounds are administered to rats and the livers quick-frozen after 30 minutes. We have completed a study which focussed on the salvage of purines: the enzymes of the metabolic pathway, the carbohydrate compounds, coenzymes, purine bases and nucleosides. The methods for measurement of enzyme activities, and the purine compounds have been newly developed and validated. Further studies are being pursued concerning the changes in free [Mg2+] in lever observed after ethanol treatment of rats as indicated by the citrate/isocitrate ratios.

嘌呤在肝脏中的代谢正在被研究，特别是在 关于乙醇施用的影响。 对男人来说，酒精 摄入或输注会导致尿酸产生升高， 高尿酸血症。这些变化伴随着醋酸盐升高和 血液中的磷酸盐浓度以及醋酸盐、磷酸盐升高 以及大鼠肝脏中焦磷酸盐的含量。 我们正在对老鼠进行研究， 在不存在或存在的情况下给予乙醇的效果 乙醇脱氢酶抑制剂； 代谢的影响 产品醋酸盐；以及磷酸盐、血浆浓度的影响， 已知其随乙醇摄入量而波动。 这些化合物 给予大鼠并在30分钟后快速冷冻肝脏。 我们完成了一项研究，重点关注嘌呤的回收： 代谢途径的酶、碳水化合物化合物、 辅酶、嘌呤碱基和核苷。 测量方法 酶活性和嘌呤类化合物已被新 开发并验证。 进一步的研究正在进行中 乙醇处理后观察到的水平中游离[Mg2+]的变化 大鼠如柠檬酸盐/异柠檬酸盐比率所示。