CHARACTERIZATION AND REGULATION OF RELEASE OF ATRIAL NATRIURETIC PEPTIDES

心房钠尿肽释放的表征和调节

• 批准号: 3822976
• 负责人: R ESKAY
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3822976
• 关键词: alcoholism /alcohol abuse; atrium; calcium; cardiovascular function; cell membrane; chemical binding; epinephrine; ethanol; high performance liquid chromatography; hormone regulation /control mechanism; human subject; immunochemistry; neurotransmitter metabolism; peptide hormone biosynthesis; pituitary gland; radioimmunoassay; saluretic; stretch receptors; vasopressins

Cardiac atria contain a family of peptides, collectively termed atrial natriuretic peptides (ANPs), which are derived from a common precursor and possess intrinsic natriuretic, diuretic, vasorelaxant and endocrine modulatory effects. Furthermore, recent immunocytochemical and radioimmunoassay (RIA) distribution studies have localized ANPs to areas of the CNS that suggest the ANPs are involved in central cardiovascular regulatory events and in the release and/or synthesis of certain pituitary gland hormones. Additional support for the possible involvement of ANPs in regulating pituitary gland function was obtained with the demonstration that high-affinity, specific ANP binding sites are present on pituitary gland membranes. Characterization by reversed phase high-pressure liquid chromatography and quantification by RIA of rat plasma ANPs revealed the presence of at least two forms of ANP ranging from 24-28 amino acids. Additional studies revealed that volume loading or pressor agents (e.g. adrenaline, vasopressin) which cause atrial stretch result in a rapid increase in circulating ANPs. The acute administration of ethanol to rats in order to achieve blood ethanol levels in the range of .2-.25% did not alter plasma levels of ANPs; however, chronic exposure of rats to ethanol vapors for 7-14 days lowered plasma ANP levels. On the basis of results obtained in vitro from cultured adult rat atrial cardiocytes, it would appear that our observed enhancement of ANPs in vivo are indirectly mediated since a number of pressor agents did not alter ANP secretion in vitro. To date only drugs or experimental conditions which alter intracellular Ca++ levels appear to modulate ANP secretion in cultured atrial myocytes.

心脏中心含有一家肽家族，统称为心房 亚钠肽（ANP），源自公共前体和 具有固有的亚钠，利尿剂，血管毛和内分泌 调节作用。 此外，最近的免疫细胞化学和 放射免疫测定（RIA）分布研究已将ANP定位于 建议ANP参与中央心血管的中枢神经系统 监管事件以及某些垂体的释放和/或合成 腺激素。 额外支持ANP的可能参与 通过演示获得调节垂体功能 垂体上存在高亲和力，特定的ANP结合位点 腺膜。 相反相高压液相色谱和反向表征 RIA对大鼠血浆ANP的定量至少存在 两种形式的ANP范围为24-28个氨基酸。 其他研究 揭示了体积加载或压力剂（例如肾上腺素， 加压素）导致心房拉伸导致快速增加 循环ANP。 急性给大鼠乙醇的急性给药 达到0.2-.25％的血液乙醇水平没有改变血浆 ANP的水平；但是，大鼠长期暴露于乙醇蒸气 7-14天降低了血浆ANP水平。 根据在以下结果中获得的结果 来自培养的成年大鼠心房心细胞的体外，看来我们 观察到体内ANP的增强是间接介导的，因为一个数字 压力剂的体外没有改变ANP的分泌。 迄今仅毒品 或改变细胞内Ca ++水平的实验条件 调节培养的心肌细胞中的ANP分泌。