GENETIC CONTROL OF DEVELOPMENTAL PATHWAYS

发育途径的遗传控制

• 批准号: 3310473
• 负责人: EDWARD B LEWIS
• 金额: $ 7.76万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-01-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3310473
• 关键词: Drosophilidae; X ray; biochemical evolution; chromosome aberrations; cytology; developmental genetics; gene deletion mutation; gene duplication; gene expression; gene interaction; genetic mapping; genetic regulatory element; genotype; growth /development; homeobox genes; phenotype; structural genes; thorax

The bithorax gene complex (BX-C) in Drosophila is a model system for studying how genes regulate growth and development. The complex spans over 300 Kb of DNA and comprises a series of protein-coding and cis-acting genetic units that act as master regulators of the growth and development of much of the thorax and abdomen of the organism. The broad objectives are (1) to determine how the individual genetic units of the complex are themselves regulated such that they become expressed along the body axis of the organism in an order that exactly parallels their order in the chromosomes; and (2) to determine the reason that the genes of the complex have remained tightly linked over the immense evolutionary time period since the cluster is believed to have arisen. The specific goals are: (1) to saturate in so far as practical cis-regulatory regions of the BX-C with chromosomal breakpoints ; (2) to characterize such breakpoints genetically, cytologically and morphologically in order to determine the specific subfunctions controlled by each cis-regulatory region; (3) to derive in so far as practical a complete series of deletions of variable length within the BX-C; and (4) to test the hypothesis that the BX-C and other similar types of gene clusters have remained intact over long evolutionary time periods as the result of a sharing of cis-regulatory regions between adjacent structural genes of the clusters. Health relatedness: Mutations within cognates of the BX-C genes in human beings may be at the basis of many types of congenital malformations and may also be responsible for many types of tumors.

果蝇中的 bithorax 基因复合体 (BX-C) 是一个模型系统 研究基因如何调节生长和发育。 该综合体横跨 300 Kb DNA，包含一系列蛋白质编码和顺式作用 作为生长和发育主要调节因子的遗传单位 生物体的大部分胸部和腹部。 广泛的目标 (1) 确定复合体的各个遗传单元如何 自身受到调节，从而沿着身体轴线表达 有机体的顺序与它们在生物体中的顺序完全平行 染色体； (2) 确定复合体基因的原因 在漫长的进化时期中一直保持着紧密的联系 因为据信该集群已经出现。 具体目标是：（1） 至 BX-C 的实际顺式调节区域饱和 染色体断点； (2) 从遗传学上表征此类断点， 细胞学和形态学以确定特异性 由每个顺式调控区域控制的子功能； (3) 推导出来 尽可能完整地删除一系列可变长度的内容 BX-C； (4) 检验 BX-C 和其他类似的假设 基因簇的类型在漫长的进化过程中保持完整 周期作为共享顺式监管区域的结果 簇的相邻结构基因。 健康相关性：突变 人类 BX-C 基因的同源基因可能是基于 许多类型的先天畸形，也可能导致许多 肿瘤类型。