GENETIC CONTROL OF DEVELOPMENTAL PATHWAYS

发育途径的遗传控制

基本信息

批准号：
3310468
负责人：
EDWARD B LEWIS
金额：
$ 16.28万
依托单位：
CALIFORNIA INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
1977
资助国家：
美国
起止时间：
1977-01-01 至 1986-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3310468
关键词：
X ray abdomen cell transformation chromatography developmental genetics gene duplication gene expression gene redundancy genetic crossing over genetic mapping genetic regulation histogenesis nucleosides radiotracer regulatory gene scanning electron microscopy thorax tissue mosaicism

项目摘要

In Drosophila, the bithorax gene complex (BX-C) is a giant cluster of genes that plays a major role in determing the body segmentation plan, especially of the thoracic and abdominal regions (Nature 276:565 570, 1978). Aims of the proposed work are to determine the mechanisms underlying cis- and trans-regulation of the complex and the nature and function of the substances produced by the genes of the complex. The specific model being tested by a combination of genetic and cytological techniques coupled with molecular approaches (the latter in a collaborative effort) is that in the wild-type organism the more posterior the segment the more genes of the complex become derepressed. Genes once derepressed in a given segment tend to be derepressed in all segments posterior thereto. The more proximal a gene is in the complex the more likely (with one exception) it is to be derepressed. Two gradients are invoked to explain the wild-type pattern: a gradient along the embryo in the Pc+ substance and a gradient along the chromosome in the affinity of the cis-regulatory elements for that substance. The long-term objective is to understand the genetic and molecular basis of a developmental pathway of a eukaryotic organism. The health significance is that the human embryo is expected to use similar sets of master regulatory genes to control the differentiation of the head, thoracic and abdominal regions of the body. Defects in such genes would be responsible for many types of congenial abnormalities. By eventually identifying such gene clusters at the molecular level, certain kinds of congenital abnormalities may eventually be corrected or alleviated.

在果蝇中，双胸基因复合体 (BX-C) 是一个巨大的基因簇这对于确定身体分割方案起着重要作用，尤其是胸部和腹部区域（Nature 276:565 570, 1978）。目标拟议的工作是确定顺式和复合物的反式调节及其性质和功能由复合体基因产生的物质。具体型号为通过遗传和细胞学技术相结合的测试分子方法（后者在合作努力中）是野生型生物，节段越靠后，其基因越多复杂的情绪变得压抑。一旦在给定片段中去抑制，基因就会倾向于其后的所有节段均被去抑制。越接近a 基因在复合体中的可能性越大（除了一个例外）去压抑。调用两个梯度来解释野生型模式： Pc+ 物质中沿胚胎的梯度和沿 Pc+ 物质的梯度染色体中顺式调节元件的亲和力物质。长期目标是了解遗传和真核生物发育途径的分子基础。这健康意义在于，人类胚胎有望使用类似的控制头部分化的主调控基因组，身体的胸部和腹部区域。这些基因的缺陷将是造成许多类型的先天性异常。最终由在分子水平上识别此类基因簇，某些种类先天性异常最终可能会得到纠正或减轻。