REGULATION OF SEX-SPECIFIC GENES IN DROSOPHILA

果蝇性别特异性基因的调控

• 批准号: 3271662
• 负责人: THOMAS W CLINE
• 金额: $ 7.74万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-06-01 至 1990-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3271662
• 关键词: Drosophilidae; alleles; autoradiography; autosomal recessive trait; bioassay; centrifugation; chromosome aberrations; developmental genetics; extrachromosomal DNA; gene frequency; gene redundancy; genetic manipulation; genetic mapping; genetic recombination; genetic regulation; genetic transcription; lethal genes; microinjections; micromanipulator; molecular cloning; molecular genetics; mutagen testing; radiotracer; regulatory gene; sex chromosomes; sex determination; species difference; temperature sensitive mutant; tissue mosaicism; transposon /insertion element

This proposal is a continuation of work designed to exploit the special features that Drosophila sex determination appears to offer for understanding how information for cellular differentiation in higher eukaryotes is genetically encoded, how it can be disrupted by mutations, and how it may have been evolved. The goal of this study is to understand the specific genetic regulatory mechanisms that operate in this organism to govern the initiation and maintenance of sex-specific pathways of development. The gene called Sex-lethal occupies a central position in this process. Tests of several hypotheses regarding Sxl's regulation are presented. One of the most important is the proposal that Sxl produces a positive self-regulatory product that is required for the initiation and/or maintenance of the gene's own activity state. Another is the proposal that a genetic element called sisterless-a is part of a system of elements whose overall relative dose serves as a developmental signal to determine Sxl's activity state very early in development. Only a 50% difference in this parameter elicits all the differences between the sexes. The genetic context in which Sxl operates will be explored, including genes that act in the mother to control, in an epigenetic fashion, the behavior of genes in the zygote. Temporal and tissue-specific aspects of Sxl's own regulation, and of its regulation of subordinate genes and/or gene products will be investigated. As a byproduct of this study of sex determination, a considerable amount should be learned regarding the effects of mobile genetic elements on gene function and meiotic recombination in higher eukaryotes. Many of the most useful mutant Sxl alleles carry insertions and rearrangements of such mobile elements. This analysis will be carried out using the techniques of developmental genetics which have proven so powerful over the past eight years of this study. This genetic work will complement a parallel study of Sxl at the molecular level, a study that was made possible by the genetic analysis that preceded it. Information from the experiments proposed here should provide the background needed to extend molecular analysis of this system into important new areas.

该提案是旨在利用特殊性的工作的延续 果蝇性别决定似乎提供的功能 了解细胞分化的信息如何在高等 真核生物是基因编码的，它如何被突变破坏， 以及它是如何进化的。 这项研究的目的是了解 在该生物体中运作的特定遗传调控机制 控制性别特异性途径的启动和维持 发展。 称为性致死（Sex-lethal）的基因在 这个过程。 关于 Sxl 调节的几个假设的检验是 提出。 其中最重要的一项是 Sxl 生产一种 启动和/或所需的积极自我监管产品 维持基因自身的活性状态。 另一个提议是 称为无姐妹-a 的遗传元素是元素系统的一部分，其 总体相对剂量作为发育信号来确定 Sxl 开发早期的活动状态。 这方面只有 50% 的差异 参数引发了性别之间的所有差异。 遗传性 将探索 Sxl 运作的背景，包括作用于 母亲以表观遗传的方式控制基因的行为 受精卵。 Sxl 自身调节的时间和组织特异性方面， 及其对从属基因和/或基因产物的调节将是 调查了。 作为这项性别决定研究的副产品， 关于移动设备的影响，我们应该了解相当多的知识 遗传元件对高等植物基因功能和减数分裂重组的影响 真核生物。 许多最有用的突变体 Sxl 等位基因都带有插入 以及此类移动元素的重新排列。 本次分析将进行 使用已被证明如此的发育遗传学技术 在过去八年的研究中，效果非常显着。 这项基因工作将 在分子水平上补充了 Sxl 的平行研究，该研究是 之前的遗传分析使其成为可能。 信息来自 这里提出的实验应该提供所需的背景 将该系统的分子分析扩展到重要的新领域。