MATERNAL CONTROL OF EMBRYONIC SEGMENTATION IN DROSOPHILA

果蝇胚胎分割的母体控制

• 批准号: 3323850
• 负责人: NORBERT PERRIMON
• 金额: $ 8.22万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1991-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3323850
• 关键词: DNA; Drosophilidae; abdomen; alleles; autoradiography; body regions; complementary DNA; developmental genetics; early embryonic stage; endonuclease; extrachromosomal DNA; gel electrophoresis; gene expression; gene interaction; gene mutation; histogenesis; laboratory mouse; laboratory rabbit; lethal genes; molecular cloning; molecular genetics; nonmammalian vertebrate embryology; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; radiation genetics; radionuclides; sex chromosomes; thorax; tissue mosaicism

We propose to combine developmental genetic and molecular approaches to analyze the roles played during Drosophila development of two late zygotic lethal loci: 1(1) dishevelled (1(1)dsh) and 1(1) hopscotch (1(1)hop). 1(1)dsh and 1(1)hop are late zygotic lethals that exhibit specific maternal effect phenotypes on embryonic segmentation. Dishevelled embryos are missing all parasegmental and segmental boundaries. Mature embryos exhibit a segment polarity phenotype. Hopscotch embryos exhibit defects in two thoracic segments (T2, T3) and abdominal segment 5 is entirely missing. 1(1)hop and 1(1)dsh are clustered on the X- chromosome at position 10B6-8. We propose to further analyze the early (maternal) and late (zygotic) effects on both hopscotch and dishevelled on embryonic development. Additionally, we will identify the DNA region of the 1(1)hop-1(1)dsh gene cluster in order to analyze the spatial and temporal expression of these genes during Drosophila development.

我们建议将发育遗传和分子结合起来 分析果蝇过程中所扮演角色的方法 两个晚期合子致死位点的发育：1(1) 蓬乱 (1(1)dsh) 和 1(1) 跳房子 (1(1)hop)。 1(1)dsh 和 1(1)hop 晚点 表现出特定母体效应表型的合子致死 关于胚胎分割。 凌乱的胚胎全部缺失 节段旁边界和节段边界。 成熟胚胎表现 片段极性表型。 跳房子胚胎出现缺陷 在两个胸段（T2、T3）和腹段 5 中 完全失踪了。 1(1)hop 和 1(1)dsh 聚集在 X- 染色体10B6-8位。 我们建议进一步分析 早期（母体）和晚期（合子）对跳房子的影响 并在胚胎发育过程中凌乱。 此外，我们将 鉴定 1(1)hop-1(1)dsh 基因簇的 DNA 区域 以便分析这些内容的空间和时间表达 果蝇发育过程中的基因。