TRiP resources for modeling human disease
用于人类疾病建模的 TRiP 资源
基本信息
- 批准号:10206288
- 负责人:
- 金额:$ 63.37万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-07-01 至 2024-03-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAffectAnimalsAreaBioinformaticsBrainCRISPR/Cas technologyClustered Regularly Interspaced Short Palindromic RepeatsCodeCollectionCommunitiesCountryDataDatabasesDepositionDiseaseDrosophila genusFundingFunding OpportunitiesGene ActivationGene ExpressionGenesGeneticGenomeGenome engineeringGeographic stateGoalsHeartHereditary DiseaseHumanIndividualInstitutesKidneyKnock-inKnock-outKnowledgeMalignant NeoplasmsMediatingMissionMonoclonal Antibody R24Neurodegenerative DisordersOrganOrthologous GenePaperPhenotypePoliciesProductionProteinsPublicationsRNA InterferenceReagentRepressionRequest for ApplicationsResearchResearch DesignResearch PersonnelResearch Project GrantsResourcesSystemTechnologyTimeTissuesTransgenic OrganismsUnited States National Institutes of Healthanimal model developmentbasecombinatorialflyfunctional genomicsgain of functiongene functiongenetic manipulationgenome-widegenomic platformhuman diseasehuman modelin vivoknock-downloss of functionmedical schoolsoverexpressionresponsesmall hairpin RNAtherapeutic targettool
项目摘要
PROJECT SUMMARY / ABSTRACT
This application requests continued funding for the TRiP, a highly successful in vivo Drosophila functional
genomics platform at Harvard Medical School. To date we have generated more than 17,000 RNAi and
CRISPR fly stocks for the research community. We propose to expand the TRiP resource of versatile and
transformative transgenic tools for gene activation, repression and genome engineering. The goals of this
resource, focused on the 3017 fly genes orthologous to genes known or suspected to be associated with
human diseases, are to: (1) generate shRNA fly stocks for RNAi that address remaining gaps in the collection,
such as genes not yet covered or reagents identified by the community as ineffective; (2) generate sgRNA
stocks for CRISPR knock out (CRISPRko) and CRISPR activation (CRISPRa); (3) Build a toolkit of reagents
that combine GAL4/UAS and a second binary expression system (LexA/lexAop or QF/QUAS) to facilitate RNAi
and/or CRISPR control of gene expression in two different tissues independently; and (5) evaluate whole
animal as well as tissue-specific loss-of-function (LOF) and gain-of-function (GOF) phenotypes associated with
the new lines and curate information on the quality of individual RNAi and sgRNA lines in our “RSVP Plus”
phenotype database. The result of these efforts will be a tremendous resource of fly stocks, distributed to the
community by the Bloomington Drosophila Stock Center, which will allow researchers to easily knock down,
knock out, or activate genes covered by the collection. When combined with the wide array of GAL4 lines, and
our proposed new collection of LexA/QF lines, this genetic toolkit will allow researchers to modulate gene
expression in any given stage and in multiple tissues simultaneously.
项目概要/摘要
该申请要求继续为 TRiP 提供资金,TRiP 是一种非常成功的果蝇体内功能
迄今为止,我们已经在哈佛医学院的基因组学平台上生成了超过 17,000 个 RNAi 和
我们建议为研究界扩展 TRiP 资源。
用于基因激活、抑制和基因组工程的转化转基因工具。
资源,重点关注 3017 个果蝇基因,这些基因与已知或疑似相关的基因同源
人类疾病的目标是:(1) 生成用于 RNAi 的 shRNA 果蝇种群,以解决收集中剩余的空白,
例如尚未涵盖的基因或被社区鉴定为无效的试剂(2)生成sgRNA;
用于 CRISPR 敲除 (CRISPRko) 和 CRISPR 激活 (CRISPRa) 的库存 (3) 构建试剂工具包;
结合 GAL4/UAS 和第二个二元表达系统(LexA/lexAop 或 QF/QUAS)以促进 RNAi
和/或 CRISPR 独立控制两个不同组织中的基因表达;和 (5) 评估整体;
动物以及组织特异性功能丧失(LOF)和功能获得(GOF)表型与
新品系并在我们的“RSVP Plus”中整理有关单个 RNAi 和 sgRNA 品系质量的信息
这些努力的结果将是一个巨大的蝇种群资源,分发给
布卢明顿果蝇库存中心社区,这将使研究人员能够轻松击倒,
当与广泛的 GAL4 系组合时,敲除或激活该集合所涵盖的基因,并且
我们提议的 LexA/QF 系新集合,该遗传工具包将允许研究人员调节基因
在任何给定阶段和多个组织中同时表达。
项目成果
期刊论文数量(0)
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会议论文数量(0)
专利数量(0)
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NORBERT PERRIMON其他文献
NORBERT PERRIMON的其他文献
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{{ truncateString('NORBERT PERRIMON', 18)}}的其他基金
Drosophila models of human mitochondrial diseases
人类线粒体疾病的果蝇模型
- 批准号:
10756280 - 财政年份:2023
- 资助金额:
$ 63.37万 - 项目类别:
Admin Core - Functional genomics resources for the Drosophila
管理核心 - 果蝇功能基因组学资源
- 批准号:
10436791 - 财政年份:2019
- 资助金额:
$ 63.37万 - 项目类别:
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