MOLECULAR ANALYSIS OF DROSOPHILA SEX DETERMINATION

果蝇性别决定的分子分析

• 批准号: 3287318
• 负责人: THOMAS W CLINE
• 金额: $ 18.36万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3287318
• 关键词: DNA; Drosophilidae; alleles; cell type; complementary DNA; cytogenetics; developmental genetics; endonuclease; gene expression; gene mutation; genetic library; genetic manipulation; genetic regulation; genetic transcription; molecular genetics; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; nucleic acid structure; regulatory gene; sex determination; transposon /insertion element

Sex-lethal is a master regulatory gene that controls all aspects of sexual dimorphism in Drosophila melanogaster. It occupies a uniquely central position in a complex, rather well-defined genetic network; indeed, it may be the most immediate site of action of the X/A balance signal which determines sex in Drosophila. It is a genetically complex locus that functions throughout all stages of development. In many important respects, the functioning of Sx1 in sex determination is analogous to that of the bithorax and Antennapedia gene complexes which determine the segmental identity of Drosophila cells. Recently these genes have been found to be homologous to genes in higher vertebrates. Characterization of such regulatory genes, and of mutations therein, seems likely to provide major insights into the mechanisms by which development is genetically programed in higher organisms, including humans, and to provide an understanding of how that programming can be upset by mutation. Sx1 has been the object of extensive genetic and developmental analysis for years. This proposal involves the first direct molecular analysis of the gene. It is designed to determine the level at which Sx1 is regulated and the mechanisms that underlie that regulation. The work should show how a single gene can encode many different product functions, and how its expression can change as a function of developmental stage, sex, and cell type. The study should contribute important new information with respect to the question of how mutations, particularly mobile genetic elements, can decrease or increase gene function. Of special interest is the characterization of a mobile element that dramatically enhances Sx1 transcript levels. The study employs modern recombinant DNA techniques to correlate Sx1 gene structure with transcript structure, in wildtype and in a wide variety of mutant situations. Developmental analysis of the Sx1 trancript pattern in wildtype and mutant individuals, combined with studies that employ P-mediated transformation by various gene constructs, should provide important tests of hypotheses generated from previous studies, and should allow us and assign specific regulatory functions to specific Sx1 transcripts. The possibility of a relationship between Drosophila sex-determining genes and those of other organisms will be explored.

性致死是一个主调控基因，控制着性的各个方面 果蝇的二态性。 它占据着得天独厚的中心位置 在一个复杂的、相当明确的遗传网络中的位置；确实，它可能 是 X/A 平衡信号最直接的作用点 决定果蝇的性别。 这是一个基因复杂的位点 功能贯穿于发展的各个阶段。 在许多重要的 在这方面，Sx1 在性别决定中的功能类似于 双胸和触角基因复合体决定了 果蝇细胞的节段同一性。 最近这些基因被 发现与高等脊椎动物的基因同源。 表征 这种调节基因以及其中的突变似乎可能提供 对遗传发育机制的主要见解 在包括人类在内的高等生物体中进行编程，并提供 了解突变如何扰乱该编程。 Sx1 有 多年来一直是广泛遗传和发育分析的对象。 该提议涉及对该基因的首次直接分子分析。 它 旨在确定 Sx1 的调节水平和 该监管的机制。 该作品应展示如何 单个基因可以编码许多不同的产品功能，以及它是如何实现的 表达可以随着发育阶段、性别和细胞的变化而变化 类型。 该研究应提供重要的新信息 突变，特别是移动遗传元件，如何能够 减少或增加基因功能。 特别令人感兴趣的是 显着增强 Sx1 的移动元件的表征 转录水平。 该研究采用现代重组 DNA 技术 将野生型和野生型中的 Sx1 基因结构与转录本结构相关联 各种各样的突变情况。 Sx1的发展分析 野生型和突变体个体的转录模式，结合研究 通过各种基因构建体采用 P 介导的转化，应该 对先前研究中产生的假设进行重要检验，并且 应该允许我们为特定的 Sx1 分配特定的监管功能 成绩单。 果蝇之间存在关系的可能性 将探索性别决定基因和其他生物体的性别决定基因。