REQUEST FOR PROTEIN/PEPTIDE SEQUENCING INSTRUMENTATION

请求蛋白质/肽测序仪器

• 批准号: 3520098
• 负责人: John E. Cronan
• 金额: $ 8万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-05-24 至 1989-05-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520098
• 关键词: biomedical equipment purchase; biomedical equipment resource; protein sequence; site directed mutagenesis

The acquisition of protein sequencing instrumentation is sought for the Genetic Engineering Facility of the Biotechnology Center at the University of Illinois. The research requiring this instrumentation is very diverse as reflected in the range of research projects that are described. Several investigators require the protein sequencer for problems involving the structure of enzyme active sites. The residue modified by site-specific reagents acting on the enzyme can be identified. Analysis of the amino acid sequence of monoclonal antibodies with differing affinities for a single hapten is required to define the components of the antigen binding site which determine the binding affinity. When combined with X-Ray crystallographic data on these antibodies, these studies will serve as the basis for future molecular modeling and genetic engineering programs to construct antibodies of defined specificity. The amino acid changes in active sites which result from site- directed mutagenesis of DNA must be confirmed by sequencing. Knowledge of N-terminal amino acid sequence of proteins and peptides are required to identify open reading frames of sequenced DNA. Often very small amounts of protein are available for these types of experiments and state-of-the-art instrumentation with sensitivity in the range of 10 pmoles or less is required. A unique application of this protein sequencer is in studies on the specificity of RNA translation in eucaryotic cells. Presence of this equipment on campus in a centralized facility will enable faculty to carry out many experiments that otherwise would be too expensive or beyond the capabilities of laboratory personnel. It also provides the most effective use of available equipment and avoids duplication of costly instrumentation in individual laboratories. The Biotechnology Center and the Graduate College will provide the laboratory space and full support facilities for operation of these instruments. On going maintenance and the presence of trained technical personnel will be assured by the Graduate College.

寻求蛋白质测序仪器的获取 用于生物技术中心的基因工程设施 在伊利诺伊大学。 需要这个的研究 仪器非常多样化，如 描述的研究项目。 几个调查人员 要求蛋白质测序仪涉及结构的问题 酶的活性位点。 由特定地点修改的残留物 可以鉴定出作用于酶的试剂。 分析 单克隆抗体的氨基酸序列不同 需要单性触觉的亲和力来定义组件 确定结合亲和力的抗原结合位点的。 当与这些上的X射线晶体学数据结合使用时 抗体，这些研究将成为未来的基础 分子建模和基因工程计划 构建定义特异性的抗体。 由位点导致的活性位点的氨基酸变化 必须通过测序确认DNA的定向诱变。 了解蛋白质的N末端氨基酸序列和 需要肽来识别开放式阅读帧 测序的DNA。 通常很少的蛋白质是 可用于这些类型的实验和最先进的 仪器的灵敏度在10 pmoles范围内 是必须的。 该蛋白序列仪的独特应用是 研究桉树细胞中RNA翻译的特异性。 该设备在集中设施的校园中存在 将使教师能够进行许多实验 太贵了或超出实验室的能力 人员。 它还提供了最有效的可用用途 设备并避免重复昂贵的仪器 个别实验室。 生物技术中心和 研究生学院将提供实验室空间和完整的空间 这些工具运行的支持设施。 去 维护和受过训练的技术人员的存在将 由研究生学院放心。