SHARED AMINO ACID ANALYZER

共享氨基酸分析仪

• 批准号: 3521772
• 负责人: RALPH G YOUNT
• 金额: $ 15.4万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521772
• 关键词: aminoacid analyzer; biomedical equipment purchase; biomedical equipment resource; protein sequence

Funds are requested to purchase a modern, state-of-the-art amino acid analyzer to be located in our Bioanalytical Center. This instrument will replace an outdated Beckman MB121 and will provide essential, more sensitive, quantitative amino acid composition data for a core of 12 users plus a wide range of other biomedically-related scientist at Washington State University and at nearby University of Idaho. Projects to be supported by this instrument include active site studies of photolabeled and photomodified contractile proteins, analysis of chemically modified and affinity labeled enzymes; characterization of endogenous peptides involved in nutrient-induced suppression of foot intake; analysis of a prostaglandin dehydrogenase in lungs and ovaries; investigation of the properties of polypeptides bound to SecB, a molecular chaperone from E coli, analysis of synthetic peptides to be used in studying the physiology and pharmacology of angiotensin receptors; characterization of enzymes and mutant enzymes which catalyze the cyclization of terpenoid substrates; characterization of newly discovered peptide hormones in plants and antigenic sites on viral proteins; quantitation of aromatic amino acids in tumors and serum.

需要资金购买现代、最先进的氨基酸 分析仪位于我们的生物分析中心。 该仪器将 取代过时的 Beckman MB121，并将提供必要的、更多的功能 12 个核心的敏感、定量氨基酸组成数据 用户以及广泛的其他生物医学相关科学家 华盛顿州立大学和附近的爱达荷大学。 项目 该仪器支持包括以下活动的现场研究 光标记和光修饰的收缩蛋白，分析 化学修饰和亲和标记的酶；的表征 内源性肽参与营养诱导的足部抑制 摄入量；肺和卵巢中前列腺素脱氢酶的分析； 研究与 SecB 结合的多肽的特性 大肠杆菌的分子伴侣，合成肽的分析 用于研究血管紧张素的生理学和药理学 受体；催化酶和突变酶的表征 萜类底物的环化；新的表征 发现植物中的肽激素和病毒上的抗原位点 蛋白质；肿瘤和血清中芳香族氨基酸的定量。