SHARED CLONING/SEQUENCING RESEARCH FACILITY

共享克隆/测序研究设施

• 批准号: 3520816
• 负责人: Jordan J Tang
• 金额: $ 10.3万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-05-16 至 1991-05-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3520816
• 关键词: aminoacid analyzer; biomedical equipment purchase; biomedical equipment resource; gel electrophoresis; molecular cloning; nucleic acid chemical synthesis; peptide chemical synthesis

Funding is requested for the purchasing of several instruments which will be part of Shared Cloning/Sequencing Research Facility (SCSRF) serving the research community at the Oklahoma Medical Research Foundation, University of Oklahoma Health Science Center, and surrounding institutions in the state of Oklahoma, including the University of Oklahoma's Norman Campus, Oklahoma State University and others. The instruments in this Research Facility are intended to support the research work of more than thirty investigators who are listed as coinvestigators of this application. The concept of the facility is to strengthen the instrumentation of medical research at the molecular level, primarily in molecular biology, protein studies, and enzymology. Excellent shared instrumentation is already present at the SCSRF, which provides instrumentations for DNA synthesis, peptide synthesis, protein microsequencing, molecular biology data base and computation, monoclonal antibody production, and computer graphic display of protein crystal structures. Currently requested instruments and the needs for them are briefly summarized below: 1. A Beckman System Gold Amino Acid Analyzer - The existing instrument at the Facility is old, insensitive, and unreliable. A replacement is needed. 2. An Applied Biosystem 380B-02 DNA Synthesizer - The existing instrument at the Facility is used to near capacity. The expansion of the research activity on this campus requires a second instrument. 3. A JASCO J-600 Spectropolarimeter - New capability to monitor for the conformation of recombinant proteins is needed. 4. An Atago Bussan Co. Stopped Flow Fast Kinetic apparatus - New capability is needed for enzymology studies, especially for comparative studies of recombinant enzyme mutants. 5. A Technology Resources MC1000 Automated Gel Scanner - New capability is needed for monitoring proteins in two-dimensional gel electrophoresis.

需要资金来购买多种仪器，这些仪器将 成为共享克隆/测序研究设施 (SCSRF) 的一部分，服务于 俄克拉荷马州医学研究基金会大学研究团体 俄克拉荷马州健康科学中心和周边机构 俄克拉荷马州，包括俄克拉荷马大学诺曼校区， 俄克拉荷马州立大学等。 本研究中的仪器 设施旨在支持三十多个研究工作 被列为本申请共同调查员的调查员。 这 该设施的概念是加强医疗仪器 分子水平的研究，主要是分子生物学、蛋白质 研究和酶学。 优秀的共享仪器已经 出席 SCSRF，该机构提供 DNA 合成仪器， 肽合成、蛋白质微测序、分子生物学数据库和 计算、单克隆抗体生产和计算机图形显示 蛋白质晶体结构。 当前请求的仪器和 他们的需求简要总结如下： 1. Beckman System Gold 氨基酸分析仪 - 现有仪器 该设施陈旧、不灵敏且不可靠。 需要更换。 2. Applied Biosystem 380B-02 DNA 合成仪 - 现有仪器 该设施已接近满负荷。 研究的扩展 该校园的活动需要第二台仪器。 3. JASCO J-600 分光旋光仪 - 监测 需要重组蛋白的构象。 4. Atago Bussan Co. Stopped Flow Fast Kinetic 装置 - 新 酶学研究需要能力，特别是比较 重组酶突变体的研究。 5. 技术资源 MC1000 自动凝胶扫描仪 - 新功能是 需要在二维凝胶电泳中监测蛋白质。