AREA DETECTOR FOR MACROMOLECULAR CRYSTALLOGRAPHY

用于高分子晶体学的区域检测器

• 批准号: 3521690
• 负责人: JOHN M ROSENBERG
• 金额: $ 26.4万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-20 至 1993-08-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3521690
• 关键词: X ray crystallography; biomedical equipment purchase; chemical structure; computer system hardware; macromolecule

The specific aim of this proposal is to purchase an area detector for macromolecular X-ray crystallography. The instrument would be installed on a rotating anode X-ray generator in the Principal Investigator's laboratory. We prefer the configuration based on a Rigaku R-AXIS II imaging plate system because it appears to be the most cost-effective system that is available at the time of this writing. The University of Pittsburgh will support this project by: Allocating $40,000 to be used as a cost sharing contribution towards the purchase of the instrument; $20,000 towards the maintenance of the equipment in the second and third years of operation (including purchase of a service contract) and half the cost of a service contract in the fourth and subsequent years (up to a limit of $8,000). This proposal is motivated by an accelerating pace of structural work in Pittsburgh that has outstripped the capacity of our existing data collection equipment. Six projects, independently funded by the NIH, currently have a total of 35 different crystal forms ready for data collection. Fourteen of these forms require screening for isomorphous derivatives while in nineteen cases only the native data need to be collected. These projects are: "Structure-Function Relationships of RNA Polymerases", "Structure-Function of Neurophysin-Hormone Systems", "StructureFunction Relationship in Glutamine Binding Protein of Escherichia coli", "Structure Characterization of Liver Aldehyde Dehydrogenase", "Structural Investigations of Mode of Action of Drugs" and "Structure and Function of EcoRI Endonuclease".

该提案的具体目标是购买一个区域探测器 大分子X射线晶体学。 仪器将被安装 首席研究员实验室的旋转阳极 X 射线发生器 实验室。 我们更喜欢基于 Rigaku R-AXIS II 的配置 成像板系统，因为它似乎是最具成本效益的 在撰写本文时可用的系统。 大学 匹兹堡将通过以下方式支持该项目： 拨款 40,000 美元用于 购买仪器的费用分摊捐款； 20,000 美元用于第二次和第三次设备的维护 运营年数（包括购买服务合同）和一半 第四年及随后几年的服务合同费用（最多 限额为 8,000 美元）。 这一提议的推动因素是结构性工作步伐的加快 匹兹堡已经超出了我们现有数据的容量 收集设备。 六个项目，由 NIH 独立资助， 目前共有35种不同的晶型可供数据使用 收藏。 其中 14 种形式需要筛选同晶型 导数，而在十九种情况下，只需要本地数据 集。 这些项目是：“RNA 的结构-功能关系 聚合酶”，“神经素-激素系统的结构-功能”， “谷氨酰胺结合蛋白的结构功能关系 大肠杆菌”，“肝醛的结构表征 脱氢酶”，“药物作用方式的结构研究” 和“EcoRI 核酸内切酶的结构和功能”。