Prototype Automated Protein Crystallization Monitoring

自动蛋白质结晶监测原型

• 批准号: 6485653
• 负责人: JOHN M ROSENBERG
• 金额: $ 18.39万
• 依托单位: XTAL MATIC, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6485653
• 关键词: X ray crystallography; biomedical automation; biomedical equipment development; computer program /software; computer system design /evaluation; crystallization; digital imaging; molecular biology information system; monitoring device; protein structure; robotics

The primary goal of this project is to build and test a prototype instrument for high throughput protein crystallography; it would automate the monitoring and storage of crystallization experiments form their initial "set up" (typically on a robot) through he final extraction for structure determination. We call this instrument a "Protein Crystallization Monitor Station" (or PCMS). It would store and monitor large number of crystallization experiment (in exchangeable cassettes of 180 microplates containint 17,280 individual experiments) at constant temperature and humidity. Crystallizations would be monitored by digital image capture; the digital image would be initially screened by machine vision software that would significantly reduce the number of images requiring human interpretation. The software associated with the PCMS would be integrated with the XtalGrow software developed in the Principal Investigator's laboratory. PROPOSED COMMERCIAL APPLICATIONS: This project will develop an automated instrument that would facilitate protein crystallization for both high-throughput and conventional protein crystallization efforts.

该项目的主要目的是建造和测试用于高通量蛋白质晶体学的原型仪器。它将通过最终提取结构确定来自动化结晶实验的监视和存储，形成其初始“设置”（通常在机器人上）。 我们将此仪器称为“蛋白质结晶监视器站”（或PCMS）。它将在恒定温度和湿度下存储和监视大量的结晶实验（在可交换的180个微板的可交换盒中包含17,280个单独的实验）。将通过数字图像捕获来监视结晶；数字图像最初将通过机器视觉软件筛选，该软件将大大减少需要人类解释的图像数量。与PCM相关的软件将与主要研究者实验室中开发的Xtalgrow软件集成。拟议的商业应用：该项目将开发一种自动化仪器，该仪器将促进蛋白质结晶，以进行高通量和常规蛋白质结晶工作。