ORAL LACTIC CULTURE SUPPLEMENTATION--FECAL BILE ACIDS AND MUTAGEN

口服乳酸菌补充剂——粪便胆汁酸和诱变剂

• 批准号: 3778163
• 负责人: DAMANNA R RAO
• 金额: --
• 依托单位: ALABAMA AGRI AND MECH UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3778163
• 关键词: African American; Lactobacillus; Streptococcus lactis; antineoplastics; beta glucuronidase; cancer prevention; cholanate compound; colon neoplasms; diet therapy; dietary supplements; enzyme mechanism; feces analysis; human subject; microorganism culture; mutagen testing; nutrition related neoplasm /cancer; nutrition related tag; oral administration; statistics /biometry; sterols; thermophilic organism

In this project the purported beneficial effects of lactic acid bacteria, commonly used in milk fermentation, in suppressing colon tumor incidence will be tested. Specifically, the hypothesis that dietary supplements of Streptococcus thermophilus and Lactobacillus acidophilus suppress the fecal mutagenic activity in humans will be tested. In parallel, the effect of these supplements on fecal bile acid and neutral sterol profile and on the fecal bacterial beta-glucuronidase activity will be studied. About 100 healthy volunteers will be screened for fecal mutagenic activity. Thirty individuals showing fecal mutagenic ratio of > 3.0 will be randomly divided into two groups of 15 each. Each group will follow a sequence of consuming: a control supplement of 4 weeks followed by lactic culture supplement (S. thermophilus or L. acidophilus) at 1010 live cells/day/subject for the next 4 weeks. At the end of each supplement 48- hour fecal samples will be collected and assayed for mutagenic activity, bile acids, neutral sterols and bacterial beta-glucuronidase activity. Also, a 4-day dietary record will be kept at the end of each 4-week period. At the end of 8-week sequence, the two groups will be switched with respect to the lactic culture supplement and another 8-week supplementation experiment will be conducted. During the experimentation, the volunteers will be instructed not to change their dietary habits drastically. Paired t test and analyses of variance will be conducted to test statistical significance. One Ph.D. level graduate student, 1 M.S. level graduate student and 1 undergraduate student will be trained in this area.

在这个项目中，乳酸菌据称具有有益作用， 常用于牛奶发酵，抑制结肠肿瘤的发生 将受到测试。 具体来说，膳食补充剂的假设 嗜热链球菌和嗜酸乳杆菌抑制粪便 将测试人类的诱变活性。 同时，效果 这些补充剂对粪便胆汁酸和中性甾醇谱以及 将研究粪便细菌β-葡萄糖醛酸酶活性。 约100 将对健康志愿者进行粪便诱变活性筛查。 三十 粪便诱变率> 3.0的个体将被随机分组 分成两组，每组 15 人。 每组将遵循以下顺序 消耗：对照补充剂 4 周，然后进行乳酸培养 补充剂（嗜热链球菌或嗜酸乳杆菌）1010 live 接下来 4 周的细胞/天/受试者。 在每个补充的末尾 48- 小时粪便样本将被收集并分析诱变活性， 胆汁酸、中性甾醇和细菌β-葡萄糖醛酸酶活性。 此外，每 4 周结束时将保存 4 天的饮食记录。 在 8 周序列结束时，两组将相互交换 补充乳酸菌和另外 8 周的补充 将进行实验。 实验过程中，志愿者 将被指示不要大幅改变饮食习惯。 配对 将进行 t 检验和方差分析来检验统计数据 意义。 一名博士。级别研究生，1 M.S.水平毕业生 学生和一名本科生将接受该领域的培训。