ANTICARCINOGENIC ACTIVITY OF LACTIC CULTURES

乳酸菌的抗癌活性

• 批准号: 3437309
• 负责人: DAMANNA R RAO
• 金额: $ 7.35万
• 依托单位: ALABAMA AGRI AND MECH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-15 至 1990-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3437309
• 关键词: azo compounds; beta glucuronidase; chemical carcinogenesis; combination cancer therapy; dietary supplements; disease /disorder model; fermentation; methylhydrazines; microorganism culture; microorganism metabolism; nitrosamines; nutrition related tag

Evidence in the literature strongly favors the hypothesis that lactic cultures commonly used in fermentation of milk suppress the formation of colon tumors. However, no controlled experiments have been conducted using animal models to test the hypothesis. Preliminary work in our laboratory (Shackelford et al., Nutr. Cancer, 5:159, 1983) indicates that lactic cultures may suppress the growth of colon carcinoma in animal models. We are proposing to study the effect of feeding supplements of Lactobacillus acidophilus and L. bulgaricus on the incidence of azoxymethane-induced intestinal tumors in rats. Groups of 40 F- 344 male rats will be assigned to 3 dietary treatments at 5 weeks of age. The diets will consist of: (1) semisynthetic diet (SSD) plus L. acidophilus supplement, (2) SSD plus L. bulgaricus supplement and (3) SSD plus uninoculated supplement (control). Supplements will provide approximately 108 viable cells/g diet. The diets will be fed for about 42 weeks. Starting at 7 weeks of age all rats will receive s/c injections of azoxymethane in saline @ 18 mg/kg body weight once a week for 2 weeks. At the end of 47 weeks of age the rats will be sacrificed (or before if moribund) and grossly examined for tumors of various organs, particularly in the small intestines, cecum, colon and rectum. The tumors will be excised, fixed in 10% neutral buffered formalin and subjected to histopathological examination for classification into adenoma and adenocarcinoma. It is hoped that this research, in addition to validating or invalidating the stated hypothesis, will create an environment for future expansion of health-related research resulting in motivation of students to choose health-related careers. An added dimension to the benefits from this research is encouraging minority students (who constitute about 90% of the enrollment at Alabama A&M University) to choose a health-related graduate education.

文献中的证据强烈支持以下假设： 常用于抑制乳汁发酵的乳酸菌 结肠肿瘤的形成。 然而，没有控制 已经使用动物模型进行了实验来测试 假设。 我们实验室的初步工作（Shackelford 等 等人，营养。 Cancer, 5:159, 1983）表明乳酸培养物可能 抑制动物模型中结肠癌的生长。 我们是 提议研究饲喂补充剂的效果 嗜酸乳杆菌和保加利亚乳杆菌对发病率的影响 氧化偶氮甲烷诱导的大鼠肠道肿瘤。 40 人一组 F- 344 只雄性大鼠将在 5 周时接受 3 次饮食治疗 的年龄。 饮食包括：(1) 半合成饮食 (SSD) 加 嗜酸乳杆菌补充剂，(2) SSD 加保加利亚乳杆菌补充剂 (3) SSD加未接种的补充剂（对照）。 补充剂 将提供大约 108 个活细胞/克饮食。 饮食会 喂养约42周。 从 7 周龄开始，所有大鼠都会 接受皮下注射氧化偶氮甲烷盐水@ 18 mg/kg身体 每周称重一次，持续两周。 47周龄结束时 老鼠将被处死（如果是垂死的，则在之前） 检查各种器官的肿瘤，特别是小器官的肿瘤 肠、盲肠、结肠和直肠。 肿瘤将被切除， 固定在 10% 中性缓冲福尔马林中并进行 组织病理学检查可分为腺瘤和 腺癌。 希望这项研究除了验证或 使所陈述的假设无效，将为 未来健康相关研究的扩展将导致 学生选择健康相关职业的动机。 一个 这项研究带来的额外好处令人鼓舞 少数民族学生（约占入学人数的 90%） 阿拉巴马农工大学）选择健康相关的毕业生 教育。