RETINAL PHARMACOLOGY OF DIRECTIONAL GANGLION CELLS

定向神经节细胞的视网膜药理学

• 批准号: 3261799
• 负责人: MICHAEL ARIEL
• 金额: $ 8.06万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-01-15 至 1987-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3261799
• 关键词: amacrine cells; catecholamine inhibitor; electron microscopy; electrophysiology; eye pharmacology; histochemistry /cytochemistry; neuroanatomy; neurotransmitters; retinal ganglion; synapses

Directionally sensitive (DS) retinal ganglion cells exist in most, if not all, vertebrate retinas. Yet little direct information is known about the retinal mechanism which generate directional sensitivity. This project proposes to study the DS cell and its amacrine cell inputs by use of intracellular and extracellular recording techniques and pharmacological manipulations. Using a superfused retinal eyecup of turtle and rabbit, the effects of synaptic transmitters/modulators and their antagonists can be tested on specific response properties of the DS cell. These results, coupled with the effects of the same agents in low Ca++/high Mg++ perfusate which blocks synaptic transmission, will determine whether certain synaptic interactions occur on the DS ganglion cell membrane. Information based on extracellular recordings and synaptic pharmacology indirectly suggests that several amacrine cell inputs to specialized regions of the ganglion cell membrane are responsible for directional sensitivity. In order to confirm the underlying wiring pattern of these DS cells, they will be stained by injection of intracellular dyes. Subsequent staining with histochemical markers for various amacrine cell naurotransmitters will visualize the interactions between these cells. Knowledge of the neural circuitry for DS cells in the mammalian retina may further the diagnostic usefulness of human optokinetic nystagmus since DS cells provide a major input to the accessory optic system which is responsible for such eye movements.

方向敏感（DS）视网膜神经节细胞存在于大多数（如果没有的话） 所有，脊椎动物的视网膜。 然而，关于该项目的直接信息知之甚少。 产生方向敏感性的视网膜机制。 这个项目 提议研究 DS 细胞及其无长突细胞输入 细胞内和细胞外记录技术和药理学 操纵。 使用海龟和兔子的灌注视网膜眼杯， 可以测试突触递质/调节剂及其拮抗剂 DS 细胞的特定响应特性。 这些结果，加上 相同药物在低 Ca++/高 Mg++ 灌注液中的作用，可阻断 突触传递，将决定某些突触相互作用是否 发生在 DS 神经节细胞膜上。 基于细胞外的信息 记录和突触药理学间接表明一些 无长突细胞输入神经节细胞膜的特殊区域 负责方向灵敏度。 为了确认 这些 DS 细胞的底层布线图案，它们将被染色 注射细胞内染料。 随后进行组织化学染色 各种无长突细胞神经递质的标记将可视化 这些细胞之间的相互作用。 了解哺乳动物视网膜中 DS 细胞的神经回路可能 自 DS 以来，进一步提高了人类视动眼球震颤的诊断价值 细胞为辅助光学系统提供主要输入 负责这种眼球运动。