PROCESSING OF BILATERAL INPUTS TO VESTIBULAR NUCLEUS

前庭核双边输入的处理

• 批准号: 6342351
• 负责人: MICHAEL ARIEL
• 金额: $ 13.92万
• 依托单位: SAINT LOUIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6342351
• 关键词: Chelonia; action potentials; afferent nerve; balance; brain stem; ear pharmacology; electrostimulus; head movements; labyrinth; lidocaine; neural information processing; neurons; neurotransmitter transport; semicircular duct; synapses; vestibular nerve; vestibular nuclei; voltage /patch clamp

DESCRIPTION: (Adapted from the Investigator's Abstract) This is a revised application (R01 03894-01A) that describes novel experiments using an in vitro brain preparation with attached temporal bones to study the initial vestibular processing of the vertebrate brainstem. The Principal Investigator initially developed these innovating techniques to study visual inputs to reflex paths that stabilize the retinal image. In this grant application, only head rotation responses will be studied in the vestibular nuclei. Whole-cell patch recordings of these responses will be examined before and after unilateral reversible lidocaine inactivation of the eighth nerve. Ipsilateral monosynaptic excitatory canal afferents converge onto neurons in the vestibular nucleus. A polysynaptic input is also thought to reach the same neurons from a contralateral canal with the corresponding axis of rotation. Vestibular nucleus neurons also display several response types during natural horizontal head rotation. Cells respond to motion to or away from the side of the recording, and their spike discharges can encode the head's velocity or acceleration. To elucidate the underlying neural circuitry of the vestibular nuclei, from the eighth nerve afferents and between the two nuclei, high-resolution patch recordings of individual synaptic events will be made as head rotation and electrical nerve stimulation evoke the excitatory and/or inhibitory pathways. Effects of synaptic drug applications will help identify the neurotransmitters involved in the monosynaptic and polysynaptic circuits. The membrane properties of the vestibular nucleus cells will be analyzed to see how they modify those synaptic inputs to yield the spike output of different vestibular response types. Redundant sensory input from both labyrinths is useful to improve vestibular sensitivity and to compensate for a unilateral loss. This project analyzes these bilateral inputs to gain an understanding of their role in the natural processing that results in a normal sense of balance or produces the feeling of vertigo during a pathological condition.

描述：（改编自研究者摘要）这是修订版 申请（R01 03894-01A）描述了使用体外 附有颞骨的大脑准备用于研究初始前庭 脊椎动物脑干的加工。首席研究员最初 开发了这些创新技术来研究反射路径的视觉输入 稳定视网膜图像。在此拨款申请中，仅头部旋转 将研究前庭核的反应。全细胞贴片记录 这些反应的一部分将在单方面可逆转之前和之后进行审查 利多卡因使第八神经失活。 同侧单突触兴奋性神经管传入神经元 前庭核。多突触输入也被认为可以达到相同的效果 来自具有相应旋转轴的对侧神经管的神经元。 前庭核神经元在自然过程中也表现出多种反应类型 头部水平旋转。细胞对向或远离一侧的运动做出反应 记录，它们的尖峰放电可以编码头部的速度或 加速度。阐明前庭的潜在神经回路 核，来自第八神经传入和两个核之间， 单个突触事件的高分辨率补丁记录将被制作为 头部旋转和电神经刺激引起兴奋和/或 抑制途径。突触药物应用的效果将有助于确定 参与单突触和多突触回路的神经递质。 将分析前庭核细胞的膜特性以了解 他们如何修改这些突触输入以产生不同的尖峰输出 前庭反应类型。 来自两个迷路的冗余感觉输入有助于改善前庭功能 敏感性并补偿单方面的损失。本项目分析 这些双边投入旨在了解它们在自然环境中的作用 产生正常平衡感或产生感觉的处理 病理状态下的眩晕。