EYE INFECTIONS BY TYPE 2 HERPES SIMPLEX VIRUS

2 型单纯疱疹病毒引起的眼部感染

基本信息

批准号：
3256032
负责人：
JANG O OH
金额：
$ 16.12万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
1976
资助国家：
美国
起止时间：
1976-09-30 至 1989-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3256032
关键词：
Alphaherpesvirinae acyclovir antibody receptor antibody specificity antiviral agents chorioretinitis herpes simplex virus 2 histopathology immunoglobulin G laboratory rabbit lymphocyte macrophage mature animal newborn animals serotyping tissue /cell culture virus classification virus cytopathogenic effect

项目摘要

The pathogenic mechanism for herpetic chorioretinitis in the neonate is poorly understood. Our preliminary studies in the newborn rabbit revealed that a) mononuclear cells infected with type 2 herpes simplex virus (HSV-2), rather than free HSV-2, produce retinochoroiditis; b) HSV-2 induced Fc receptors for normal IgG on mononuclear cells; and c) mononuclear cells infected with HSV-2 required normal IgG for the induction of the ocular lesion in the newborn rabbits. Based on these observations, as well as those of other researchers, the following pathogenic process for the development of retinochoroiditis in the neonate is proposed: Following infection, HSV-2 induces Fc receptors for normal IgG on the mononuclear cells. The binding of IgG to the Fc receptors changes the nature of the cell surface of HSV-infected mononuclear cells and leads to the entrapment of the cell in the capillaries of the retina and choroid. The entrapped HSV-infected mononuclear cell then initiates infection at the site and produces retinochoroiditis. To examine the validity of this proposed pathogenic process, the following series of experiments will be carried out: a) subpopulations of mononuclear cells which are major carriers of HSV-2 and are capable of producing retinochoroiditis in the newborn rabbits will be determined; b) the interaction between HSV-2 and mononuclear cells will be studied, and HSV-induced Fc receptors on the mononuclear cells will be characterized; c) the effects of binding of IgG to HSV-induced Fc receptors on the HSV-infected mononuclear cells and on the induction of retinochoroiditis will be evaluated. Finally, (d) the effect of HSV-specific antibody and of the antiviral agents on the HSVG-infected mononuclear cells will be tested. If the validity of this hypothesis can be proven, our investigation will have important medical implications: a) The pathogenic process for neonatal herpetic retinochoroiditis will be clarified, and an effective means of preventing and treating the disease will be found; b) the biological role of Fc receptors in the pathogenesis of herpetic retinochoroiditis will be identified; and c) the findings of this investigation will shed some light in our understanding of pathogenesis of retinochoroiditis in the newborn produced by other viruses, such as varicella-zoster, cytomegalo, measles and rubella viruses.

新生儿疱疹性脉络膜视网膜炎的发病机制是不太了解。我们对新生兔子的初步研究表明 a) 感染2型单纯疱疹病毒的单核细胞 (HSV-2)，而不是游离的 HSV-2，会产生视网膜脉络膜炎； b) HSV-2 在单核细胞上诱导正常 IgG 的 Fc 受体；和c) 感染 HSV-2 的单核细胞需要正常 IgG 进行诱导新生兔的眼部病变。根据这些观察，以及其他研究人员的研究表明，以下致病过程提出新生儿视网膜脉络膜炎的发展：感染后，HSV-2 诱导单核细胞上正常 IgG 的 Fc 受体细胞。 IgG 与 Fc 受体的结合改变了 Fc 受体的性质 HSV 感染的单核细胞的细胞表面并导致包埋视网膜和脉络膜毛细血管中的细胞。被困的然后，HSV 感染的单核细胞在该部位开始感染，产生视网膜脉络膜炎。为了检验所提出的致病过程的有效性，以下内容将进行一系列实验：a) 单核细胞是 HSV-2 的主要携带者，能够确定新生兔是否发生视网膜脉络膜炎； b) 将研究 HSV-2 和单核细胞之间的相互作用，以及将表征单核细胞上 HSV 诱导的 Fc 受体； c) IgG 与 HSV 诱导的 Fc 受体结合对 HSV感染的单核细胞及其对视网膜脉络膜炎的诱导将被评估。最后，(d) HSV 特异性抗体和将测试 HSVG 感染的单核细胞上的抗病毒药物。如果这个假设的正确性能够被证明，我们的调查将会具有重要的医学意义： a) 的致病过程新生儿疱疹性视网膜脉络膜炎将得到澄清，并寻找有效的治疗方法将找到预防和治疗该疾病的方法； b) 的 Fc受体在疱疹发病机制中的生物学作用将确定视网膜脉络膜炎； c) 本研究的结果调查将有助于我们了解疾病的发病机制由其他病毒引起的新生儿视网膜脉络膜炎，例如水痘带状疱疹病毒、巨细胞病毒、麻疹病毒和风疹病毒。