IMAGE PROCESSING IN PRENATAL ANALYSIS

产前分析中的图像处理

• 批准号: 3500090
• 负责人: DAVID H BING
• 金额: $ 5万
• 依托单位: CBR LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3500090
• 关键词: blood tests; cell cycle; centromere; child (0-11); cytogenetics; female; fluorescent dye /probe; human subject; image processing; in situ hybridization; karyotype; leukocytes; mathematical model; method development; nucleic acid probes; nucleic acid repetitive sequence; prenatal diagnosis; statistics /biometry; tissue /cell culture; trophoblast; video recording system

For prenatal cytogenetic analysis, cells are now sampled from the amniotic sac, a procedure which carries some risk and some women will not submit to it. There is a desire to find a less invasive and less risky sampling procedure. A number of laboratories are developing procedures to enrich fetal trophoblasts from maternal blood. These procedures are not expected to provide pure populations of fetal cells, so a means of verifying fetal origin for female fetuses is needed. To verify fetal origin, we propose to develop a statistics-based image analysis of metaphase chromosomes labelled with fluorescent alpha satellite DNA probes. We base our strategy on the fact that the number of copies of alpha-satellite DNA repeats is variable in human populations, so an analysis of intensity of the fluorescent "light stars" emanating from each chromosome should allow us to distinguish fetal from maternal cells. This procedure uses the same metaphase spreads prepared for standard prenatal cytogenetics, so standard analysis can be carried out on previously stored images of the fetal-verified cells. A simpler version of these procedures will provide a means for uniquely identifying humans for paternity and forensic purposes.

对于产前细胞遗传学分析，现在从 羊膜囊手术存在一定风险，有些女性不会 服从它。 人们渴望找到一种侵入性较小、风险较小的方法 抽样程序。 许多实验室正在开发程序 从母体血液中丰富胎儿滋养层。 这些程序是 预计不会提供纯胎儿细胞群，因此一种方法 需要验证女性胎儿的胎儿来源。 为了验证胎儿起源，我们建议开发基于统计的图像 荧光α标记的中期染色体分析 卫星 DNA 探针。 我们的战略基于以下事实： α-卫星 DNA 重复序列的拷贝数在人类中是可变的 人口，因此对荧光“光星”的强度进行分析 来自每条染色体的信息应该能够让我们区分胎儿和胎儿 母体细胞。 该程序使用为标准准备的相同中期扩散 产前细胞遗传学，因此可以进行标准分析 先前存储的胎儿验证细胞的图像。 更简单的版本 这些程序将提供一种唯一识别人类的方法 用于亲子鉴定和法医目的。