SIMPLIFIED FROZEN BLOOD METHODOLOGY FOR AUTOLOGOUS BLOOD

自体血的简化冷冻血液方法

• 批准号: 3508724
• 负责人: DAVID H BING
• 金额: $ 20.56万
• 依托单位: CBR LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3508724
• 关键词: adenosine triphosphate; autotransfusion; blood preservation; cryopreservation; diphosphoglycerate; erythrocytes; evaluation /testing; freezing; human tissue; temperature; thermostability

DESCRIPTION (Investigator's Abstract): The goal of this Phase II project is to determine whether frozen red cells stored at 25 degrees Celsius for periods up to six months will have an in vivo survival equal to that of frozen red cells stored at -65 degrees Celsius for periods up to ten years, and will meet the criteria established by the Food and Drug Administration Office of Biological Research and Review for the blood product, "red blood cells frozen." During a Phase 1 project, several methods of glycerolizing red blood cells prior to freezing at -25 degrees Celsius were explored and methods of thawing these cells were also evaluated. Preliminary data from Phase 1 suggested that greater than 90 percent of red cell recovery could be achieved after -25 degrees Celsius storage for up to three months. The specific aim of the Phase 2 proposal is to evaluate stability of red blood cells cryopreserved by these methods over a twelve-month period. Red cell survival and recovery after transfusion will be assessed as well as biochemical characteristics, including 2/3 DPG and ATP levels to evaluate the products suitability for transfusion. The overall goal of the study is to develop technology to expand the capabilities of autologous blood donor programs to include the use of a simplified method for freezing preservation at - 25 degrees Celsius. The commercial potential of such a development would be considerable, in that institutions lacking sophisticated freezing and processing equipment for dealing with frozen blood requests could participate in this activity. Probably the most relevant application for this country would be in the area of autologous blood collection. Routine access to blood freezing technology would expand the interval between the time the first unit is collected and the time of the electric surgery. It may also have application in underdeveloped countries lacking sophisticated freezing equipment for maintenance of rare blood units.

描述（研究者摘要）：第二阶段项目的目标 是为了确定冷冻红细胞是否可以在25摄氏度下保存 长达六个月的体内存活率与 冷冻红细胞在-65摄氏度下保存长达十年， 并将符合食品和药物管理局制定的标准 生物研究和审查办公室血液制品“红血 细胞冷冻。”在第一阶段项目中，几种甘油化方法 对在 -25 摄氏度下冷冻之前的红细胞进行了探索并 还评估了解冻这些细胞的方法。 初步数据来自 第一阶段表明，超过 90% 的红细胞恢复率可以 -25摄氏度储存长达三个月后即可达到。 这 第二阶段提案的具体目标是评估红血的稳定性 通过这些方法将细胞冷冻保存十二个月。 红细胞 将评估输血后的存活率和恢复情况 生化特征，包括 2/3 DPG 和 ATP 水平进行评估 产品是否适合输血。 研究的总体目标是 开发技术以扩大自体献血者的能力 计划包括使用简化的冷冻方法 保存于零下25摄氏度。 这样的商业潜力 发展将是相当可观的，因为缺乏机构 用于处理冷冻食品的先进冷冻和加工设备 有献血需求的可以参加此活动。 可能是最多的 该国的相关应用将在自体移植领域 血液采集。 血液冷冻技术的常规使用范围将扩大 收集第一个单元的时间与收集第一个单元的时间之间的间隔 电动手术。 在欠发达地区也可能有应用 缺乏先进冷冻设备来维护稀有资源的国家 血液单位。