CRITICAL FLUID EXTRACTION AND PURIFICATION OF TAXOL

紫杉醇的临界流体萃取和纯化

• 批准号: 3492967
• 负责人: TREVOR P. CASTOR
• 金额: $ 5万
• 依托单位: APHIOS CORPORATION
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-30 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3492967
• 关键词: antineoplastics; antiviral agents; biological products; chemical synthesis; chromatography; diterpenes; drug design /synthesis /production; evaluation /testing; fluid; paclitaxel; plant extracts

DESCRIPTION: (Adapted from the applicant's abstract) Numerous compounds showing antineoplastic activity have been identified in the organic solvent extracts of plant material, microorganisms and marine organisms. Further investigation into the utility of these compounds as chemotherapeutic and anti-HIV agents has been hampered by their low natural abundance and the inefficiencies of conventional extraction techniques. An example of one such compound is taxol (NSC 125973), a diterpene plant product derived from the western yew Taxus brevifolia. The conventional organic phase extraction and chromatographic purification process for preparing taxol is time consuming and could also be harmful to the biologically active compounds because of their long residence time in a harsh environment. This technique is also encumbered by low overall yields. The specific aims of this application are to investigate the application of supercritical fluid extraction and chromatography to the isolation and purification of natural products with anticancer or anti-HIV activity.

描述：（改编自申请人的摘要）多种化合物 在有机溶剂中已鉴定出具有抗肿瘤活性 植物材料、微生物和海洋生物的提取物。 更远 研究这些化合物作为化疗药物的效用 抗艾滋病毒药物的天然丰度较低且缺乏 传统提取技术效率低下。 一个例子 这种化合物是紫杉醇 (NSC 125973)，一种二萜植物产品，源自 西部红豆杉 短叶红豆杉。 常规有机相 提取色谱纯化制备紫杉醇的工艺流程为 耗时且还可能对生物活性有害 化合物，因为它们在恶劣环境中的停留时间较长。 该技术还受到总产量低的阻碍。 具体目标 本应用的目的是研究超临界的应用 流体萃取和色谱法的分离和纯化 具有抗癌或抗艾滋病毒活性的天然产物。