GLYCOSYLATION AND GLYCOSIDASES - CELL AND MOL. BIOLOGY

糖基化和糖苷酶 - 细胞和分子。

• 批准号: 3484716
• 负责人: PHILLIPS W ROBBINS
• 金额: $ 29.66万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-02-01 至 1993-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3484716
• 关键词: Candida albicans; N glycosidase; O glycosidase; Saccharomyces; asparagine; beta glucosidases; cell wall; chitin; dolichol; electron microscopy; fungal genetics; gene complementation; glucans; glycosylation; glycosyltransferase; laboratory rabbit; mannosidase; molecular cloning; protein biosynthesis; protein sequence; restraint; temperature sensitive mutant

This proposal focuses on the biological functions of glycosylation and glycosidases and much of the work is concerned with the yeast cell wall. We have recently shown that the classical "chitin synthase" of Saccharomyces is not the enzyme involved in budding but that a second enzyme, hitherto undetected, is responsible for bud scar formation. We will investigate biochemical and molecular aspects of this second system. We consider the interesting spatial distribution of chitin in budding yeast to be an intriguing problem in "development". Since starting our exploration of chitin deposition, we have been attracted by other biological aspects of the yeast cell wall. For example, the complex process of mating with its changes in cell shape and cell wall composition, and the timing restraints imposed on cell wall deposition by the cell cycle have suggested numerous biochemical and molecular experiments. Most of the work outlined in this proposal relate directly to these themes.

该提案重点关注糖基化的生物学功能 和糖苷酶，大部分工作都与酵母有关 细胞壁。 我们最近表明，经典的“甲壳素 酵母菌的“合成酶”不是参与出芽的酶 但迄今为止尚未发现的第二种酶负责 芽痕形成。 我们将研究生物化学和分子 第二个系统的各个方面。 我们考虑有趣的空间 芽殖酵母中几丁质的分布是一个有趣的问题 在“发展”中。 自从开始探索甲壳素沉积以来，我们一直在 被酵母细胞壁的其他生物学方面所吸引。 为了 例如，复杂的交配过程及其细胞的变化 形状和细胞壁组成，以及施加的时间限制 关于细胞周期的细胞壁沉积已经提出了许多 生化和分子实验。 大部分工作概述 本提案中的内容与这些主题直接相关。