EVOLUTION AND DIVERSITY OF EUKARYOTIC GLYCOSYLATION

真核糖基化的进化和多样性

• 批准号: 7602028
• 负责人: PHILLIPS W ROBBINS
• 金额: $ 0.65万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-03 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602028
• 关键词: Bioinformatics; Computer Retrieval of Information on Scientific Projects Database; Dolichol; Entamoeba histolytica; Evolution; Funding; Giardia lamblia; Golgi Apparatus; Grant; Human; Institution; Leishmania; Link; Modeling; Modification; Oligosaccharides; Polysaccharides; Protein Glycosylation; Proteins; Proteome; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Source; Trichomonas vaginalis; Trypanosoma; United States National Institutes of Health; base; genome sequencing; glycosylation; positional cloning; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. While Saccharomyces cerevisiae has been a good model for human N- and O-linked glycosylation and GPI-anchor synthesis, most of the evolution and diversity in protein glycosylation exists among deeply divergent protists. Glycosylation of kinetoplastids (e.g. Trypanosoma sp. and Leishmania sp.) has been extensively studied, but glycosylation of microaerophilic protists (Trichomonas vaginalis, Giardia lamblia (GI), and Entamoeba histolytica (Eh) are not well studied. Whole genome sequences that predict the total proteins (proteome) are now available for all of these protist species. Preliminary evidence of the diversity of eukaryotic glycosylation based upon our bioinformatics/ experimental approach predict that Eh and Tv make abbreviated dolichol-linked N-glycan precursors. Preliminary experiments demonstrate that Eh and Tv do, indeed, synthesize abbreviated dolichol-linked N-glycan precursors. Therefore, the truncated precursors will likely be the starting point for any subsequent modifications. However, reverse genetics may not predict modifications that are specific to the niche of a given protest and, therefore, structural studies will be conducted to determine if the oligosaccharides are modified by additional Golgi or Golgi-like modifications or if they are simply truncated as found in, Trypanosomes.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 尽管酿酒酵母是人类N和O-连接糖基化和GPI锚合成的良好模型，但在深层差异的生物中，蛋白质糖基化的大部分进化和多样性都存在。 已经进行了广泛的研究，但已经进行了广泛的研究，但微粒细胞的糖基化（trichomonas vaginalis，giardia lamblia（gi）和Entamoeba Histolya（EH）是概述的概念（EH），整个概念是概念的，糖基糖基（EH）并没有很好地预测，糖基糖基的整体蛋白质（EH）是预测的，糖基糖基的整体是预测的。现在可以根据我们的生物信息学/实验方法来预测EH和TV可以使缩写与Dolichol链接的N-聚糖前体的前体可预测所有这些原生物种的初步证据。 初步实验表明，EH和TV确实合成了缩写的Dolichol连接的N-聚糖前体。 因此，截断的前体可能是任何后续修改的起点。 但是，反向遗传学可能无法预测特定于给定抗议的细分市场的修改，因此，将进行结构研究，以确定寡糖是否通过其他高尔基（Golgi）或类似高尔基（Golgi）的修饰进行了修饰，或者是否仅根据锥虫（Trypanosomes）的发现而被删除。