HEMORRHAGIC AND NECROTIC TOXINS IN SNAKE VENOMS

蛇毒中的出血性和坏死性毒素

• 批准号: 3484196
• 负责人: ANTHONY T TU
• 金额: $ 13.02万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-06-01 至 1992-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3484196
• 关键词: Raman spectrometry; adenosinetriphosphatase; animal poison; antidotes; atomic absorption spectrometry; chemical structure function; collagenase; conformation; dialysis; drug metabolism; electrofocusing; electron microscopy; enzyme mechanism; erythrocyte membrane; gel electrophoresis; gel filtration chromatography; hemorrhage; high voltage electrophoresis; histology; hyaluronidase; ion exchange chromatography; kidney necrosis; laboratory mouse; membrane model; metals; microscopy; muscle necrosis; necrosis; peptidases; platelet aggregation; proteolysis; reptile poison; sarcoplasmic reticulum; snakes; toxin; zinc

Snakebite is a common occurrence in many parts of the world, paticularly in tropical ares. The venoms of certan snakes, especially the Crotalid family, which includes rattlesnakes, cooperheads, and water mocassins in the United States, causes extensive tissue damage, which in many cases leads to dysfunction or complete loss of extremity. Our objective is to determine the chemical and pharmavological properties of the toxins responsible for hemorrhage and necrosis and the mechanisms of tissue damage on envenomation. Different hemorrhagic toxins and myotoxins will be isolated from snake venoms and their effects on artificial membranes, platelet aggregation, isolated endothelial cells, and sarcoplasmic reticulum will be investigated. In order to establish structure- function relationships of myotoxin a, the toxin wll be modified using several methods and its effect on myonecrotic activity and SR calcium-ATPase activity will be investigated. For the long-term goal, we hope that our research will contribute to the long-range objective of better snakebite treatment, especially n the prevention of tissue damage. Also, by developing the experimental model for this research, we predict their applicability to the study of myodegenerating afflictions and hemorrhagic disorders of man.