HEMORRHAGIC AND NECROTIC TOXINS IN SNAKE VENOMS

蛇毒中的出血性和坏死性毒素

• 批准号: 3484197
• 负责人: ANTHONY T TU
• 金额: $ 16.83万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-06-01 至 1997-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3484197
• 关键词: Raman spectrometry; animal poison; antidotes; atomic absorption spectrometry; calcium transporting ATPase; chemical structure function; collagenase; conformation; dialysis; drug metabolism; electrofocusing; electron microscopy; enzyme mechanism; erythrocyte membrane; gel electrophoresis; gel filtration chromatography; hemorrhage; high voltage electrophoresis; histology; hyaluronidase; ion exchange chromatography; kidney necrosis; laboratory mouse; membrane model; metals; microscopy; muscle necrosis; necrosis; peptidases; platelet aggregation; proteolysis; reptile poison; sarcoplasmic reticulum; snakes; toxin; zinc

Snakebite is a common occurrence in many parts of the world, paticularly in tropical ares. The venoms of certan snakes, especially the Crotalid family, which includes rattlesnakes, cooperheads, and water mocassins in the United States, causes extensive tissue damage, which in many cases leads to dysfunction or complete loss of extremity. Our objective is to determine the chemical and pharmavological properties of the toxins responsible for hemorrhage and necrosis and the mechanisms of tissue damage on envenomation. Different hemorrhagic toxins and myotoxins will be isolated from snake venoms and their effects on artificial membranes, platelet aggregation, isolated endothelial cells, and sarcoplasmic reticulum will be investigated. In order to establish structure- function relationships of myotoxin a, the toxin wll be modified using several methods and its effect on myonecrotic activity and SR calcium-ATPase activity will be investigated. For the long-term goal, we hope that our research will contribute to the long-range objective of better snakebite treatment, especially n the prevention of tissue damage. Also, by developing the experimental model for this research, we predict their applicability to the study of myodegenerating afflictions and hemorrhagic disorders of man.

蛇咬伤在世界许多地方都很常见， 特别是在热带地区。 某些蛇的毒液， 尤其是响尾蛇科，其中包括响尾蛇， 美国的库珀头和水莫卡辛，导致 广泛的组织损伤，在许多情况下会导致功能障碍 或肢体完全丧失。 我们的目标是确定化学和药理学 导致出血和坏死的毒素的特性 以及毒液造成组织损伤的机制。 不同的 从蛇体内分离出出血毒素和肌肉毒素 毒液及其对人工膜、血小板的影响 聚集、分离的内皮细胞和肌浆 网状结构将被调查。 为了建立结构- 肌毒素a的功能关系，毒素将被修饰 使用几种方法及其对肌坏死活动的影响 将研究 SR 钙-ATP 酶活性。 为了长远目标，我们希望我们的研究能够做出贡献 为了更好地治疗蛇咬伤的长期目标， 尤其是预防组织损伤。 此外，通过开发本研究的实验模型，我们 预测它们在肌变性研究中的适用性 人类的痛苦和出血性疾病。