COMPARISON OF SPINAL AND SUPRASPINAL PAIN THRESHOLDS

脊柱和脊柱上疼痛阈值的比较

• 批准号: 3477822
• 负责人: GEORGE S. BORSZCZ
• 金额: $ 11.06万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3477822
• 关键词: analgesia; laboratory rat; model design /development; morphine; neurophysiology; operant conditionings; pain; pain threshold; spinal cord; analgesia; laboratory rat; model design /development; morphine; neurophysiology; operant conditionings; pain; pain threshold; spinal cord

The study describes a behavioral paradigm, using the rat, that allows direct comparison of The study describes a behavioral paradigm, using the mt, thresholds of nociceptive responses organized at spinal and supraspinal levels, and thereby permits determination of the relative efficacy of hypoalgesic treatments on these response systems. During testing, the rat's tail flick reflex is monitored while the animal performs an operant wheel turn response to escape current applied to the tail Thresholds for tail flick and wheel turn escape are assessed by the psychophysical method of constant stimuli. This paradigm is also designed to determine whether changes in response thresholds are confounded by changes in motor performance. Part 1 of the study employs the wheel turn / tail flick paradigm to assess the relative influence of a variety of hypoalgesic treatments on spinal and supraspinal pain thresholds. The treatments chosen for study are those that have been shown in the human literature to dissociate the affective-motivational and sensory-intensity dimensions of the pain experience. These treatments include systemic administration of the narcotics morphine and fentanyl, and of the benzodiazepine diazepam. Also included is presentation of a warning signal prior to application of the noxious stimulus. It is argued that ff the dissociative effects of these treatments on the dimensions of human pain are reflected by differential increases in wheel turn and tail flick thresholds, then the animal paradigm may provide a model system for the study of human pain sensitivity. Given a viable animal model, it is hoped that information garnered from the study of underlying neural mechanisms of nociception and hypoalgesia will more readily generalize to human experimental and clinical studies. Part 2 examines the underlying mechanisms that account for the dissociative effects of narcotic analgesics. Experiments in Part 2 focus on the means by which morphine administered either intracerebrally or intrathecally differentially raise spinal and supraspinal response thresholds. In order to classify the mechanisms by which these treatments dissociate response thresholds the effects of intracerebral and/or intrathecal administration of morphine, monoamines, purines and their antagonists are examined using the wheel turn tail flick paradigm.

该研究描述了使用大鼠的行为范式，该范式允许 该研究的直接比较描述了行为范式，使用 MT，在脊柱和脊柱上组织的伤害感受反应的阈值 水平，从而允许确定相对疗效 这些响应系统上的低相和治疗。在测试过程中，老鼠的 在动物执行操作轮时监视尾部轻弹反射 转动响应以逃避电流应用于尾部阈值 轻弹和车轮转弯逃脱通过心理物理方法评估 恒定刺激。该范式还旨在确定是否 响应阈值的变化被电机的变化混淆 表现。 该研究的第1部分采用车轮转弯 /尾部轻弹范式来评估 多种低凝治疗对脊柱和脊柱的相对影响 脊柱上疼痛阈值。选择学习的治疗方法是 在人类文献中已显示以解离 疼痛的情感动机和感觉强度尺寸 经验。这些治疗包括全身管理 麻醉品吗啡和芬太尼，以及苯二氮卓的地西epa。还 其中包括在应用之前的警告信号的介绍 有害刺激。有人认为，ff这些的分离效应 差分反映了人类痛苦方面的治疗 车轮转弯和尾部轻弹阈值增加，然后是动物范式 可以为研究人类疼痛敏感性提供模型系统。给定 可行的动物模型，希望从研究中获得的信息 潜在的伤害感受和性低敏性神经机制将更多 容易概括到人类的实验和临床研究。 第2部分研究了解离的基本机制 麻醉镇痛药的影响。第2部分中的实验专注于手段 吗啡在脑内或胸前施用 差异提高脊柱和脊柱上响应阈值。为了 为了分类这些治疗分解反应的机制 阈值脑内和/或鞘内给药的影响 使用吗啡，单胺，嘌呤及其拮抗剂，使用 车轮转动范围范式。