COMPARISON OF SPINAL AND SUPRASPINAL PAIN THRESHOLDS

脊柱和脊柱上疼痛阈值的比较

• 批准号: 3477822
• 负责人: GEORGE S. BORSZCZ
• 金额: $ 11.06万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3477822
• 关键词: analgesia; laboratory rat; model design /development; morphine; neurophysiology; operant conditionings; pain; pain threshold; spinal cord

The study describes a behavioral paradigm, using the rat, that allows direct comparison of The study describes a behavioral paradigm, using the mt, thresholds of nociceptive responses organized at spinal and supraspinal levels, and thereby permits determination of the relative efficacy of hypoalgesic treatments on these response systems. During testing, the rat's tail flick reflex is monitored while the animal performs an operant wheel turn response to escape current applied to the tail Thresholds for tail flick and wheel turn escape are assessed by the psychophysical method of constant stimuli. This paradigm is also designed to determine whether changes in response thresholds are confounded by changes in motor performance. Part 1 of the study employs the wheel turn / tail flick paradigm to assess the relative influence of a variety of hypoalgesic treatments on spinal and supraspinal pain thresholds. The treatments chosen for study are those that have been shown in the human literature to dissociate the affective-motivational and sensory-intensity dimensions of the pain experience. These treatments include systemic administration of the narcotics morphine and fentanyl, and of the benzodiazepine diazepam. Also included is presentation of a warning signal prior to application of the noxious stimulus. It is argued that ff the dissociative effects of these treatments on the dimensions of human pain are reflected by differential increases in wheel turn and tail flick thresholds, then the animal paradigm may provide a model system for the study of human pain sensitivity. Given a viable animal model, it is hoped that information garnered from the study of underlying neural mechanisms of nociception and hypoalgesia will more readily generalize to human experimental and clinical studies. Part 2 examines the underlying mechanisms that account for the dissociative effects of narcotic analgesics. Experiments in Part 2 focus on the means by which morphine administered either intracerebrally or intrathecally differentially raise spinal and supraspinal response thresholds. In order to classify the mechanisms by which these treatments dissociate response thresholds the effects of intracerebral and/or intrathecal administration of morphine, monoamines, purines and their antagonists are examined using the wheel turn tail flick paradigm.

该研究描述了一种使用老鼠的行为范式，它允许 直接比较 该研究描述了一种行为范式，使用 mt，脊髓和脊髓上组织的伤害性反应阈值 水平，从而可以确定相对功效 对这些反应系统进行镇痛治疗。在测试过程中，老鼠的 当动物进行操作轮时，监测甩尾反射 对施加到尾部的逃逸电流的转向响应 尾部阈值 轻弹和转轮逃生通过心理物理学方法进行评估 持续的刺激。该范例还旨在确定是否 响应阈值的变化与运动的变化相混淆 表现。 研究的第 1 部分采用车轮转动/甩尾范式来评估 各种镇痛治疗对脊柱和神经系统的相对影响 脊髓上疼痛阈值。选择用于研究的治疗方法是 人类文献已表明可以分离 疼痛的情感动机和感觉强度维度 经验。这些治疗包括全身给药 麻醉剂吗啡和芬太尼，以及苯二氮卓类地西泮。还 包括在应用之前发出警告信号 有害刺激。有人认为，ff 这些的解离效应 对人类痛苦维度的治疗通过差异化来体现 车轮转动和甩尾阈值增加，然后是动物范式 可能为人类疼痛敏感性的研究提供一个模型系统。给定一个 可行的动物模型，希望从研究中获得的信息 伤害感受和痛觉减退的潜在神经机制将更多 很容易推广到人体实验和临床研究。 第 2 部分探讨了解离的根本机制 麻醉镇痛药的作用。第 2 部分中的实验重点关注以下方法： 吗啡是脑内注射还是鞘内注射 差异性地提高脊髓和脊髓上反应阈值。为了 对这些治疗分离反应的机制进行分类 脑内和/或鞘内给药效果的阈值 使用吗啡、单胺、嘌呤及其拮抗剂进行检查 车轮转尾轻弹范例。