CEREBELLAR-BRAINSTEM CIRCUITS & CONDITIONED BRADYCARDIA

小脑脑干回路

• 批准号: 3475579
• 负责人: WILLIAM F SUPPLE
• 金额: $ 8.12万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3475579
• 关键词: afferent nerve; amygdala; autogenic training; bradycardia; brain mapping; brain regulatory center; brain stem; cerebellar Purkinje cell; cerebellum; experimental brain lesion; fear; heart innervation; laboratory rabbit; memory; neuroanatomy; neurophysiology; neurotoxins; parabrachial nucleus

The search for the biological alterations that occur in the brain that are substrates for learning and memory has proved to be one of the most elusive in science. Several important discoveries have led to the initial localization of neural circuits putatively involved in certain classically conditioned responses. Recently,convergent lesion/behav- ioral, electrophysiological and anatomical data have established the midline cerebellum as essential for the acquisition and retention of conditioned bradycardia in the rabbit. The proposed research is based on the hypothesis that the midline cerebellum, along with the central n. of the amygdala (ACE), are essential components of a more extensive neuro- anatomical system involved in the acquisition and retention of this autonomic conditioned response. We hypothesize it is through anatomical connections with brainstem cardioregulatory nuclei that the midline cerebellum makes these essential functional contributions to conditioned bradycardia. In keeping with this hypothesis, the objective of the present research is to further our understanding of the functional contributions of the cerebellum to autonomic conditioning by studying the anatomical and neurophysiological interactions between the midline cerebellum and the pontine parabrachial n. (PBN), a brainstem cardioregulatory n. with (i) direct anatomical connections with the midline cerebellum an( (ii) direct anatomical connections with a forebrain n. importantly involved in conditioned bradycardia, the ACE. Toward this objective, the present research has been designed to (a) determine precisely the anatomical relationships between the midline cerebellum and the PBN, (b) characterize the neurophysiological responses and interactions between neurons in these regions, and the alterations which may occur as a result of aversive Pavlovian conditioning and, (c) the effects of neurotoxic lesions of components of this cerebellar-parabrachial circuit on the acquisition and retention of conditioned bradycardia in the rabbit. These data will considerably extend our knowledge of the manner in which cerebellar neurons interact with other brain regions during conditioned bradycardia, and enhance our understanding of brain function by identifying, more exactly, a specific brain system involved in learning and memory. Such an identification is a necessary prerequisite to a more complete understanding of the ultrastructural and molecular substrates of learning and memory; processes basic to the survival of the organism. These data will be exciting given very recent evidence of cerebellar involvement in complex human learning and emotional processes.

寻找大脑中发生的生物学改变 被证明是学习和记忆的基板是最多的 在科学方面难以捉摸。 几个重要发现导致了 神经回路的最初定位与某些人相关 经典的响应。 最近，收敛性病变/行为 - Ioral，电生理和解剖学数据已经确定了 中线小脑对于获取和保留至关重要 在兔子中有条件性心动过缓。 拟议的研究是基于 中线小脑以及中央n的假设。的 杏仁核（ACE）是更广泛的神经 - 涉及获得和保留的解剖系统 自主条件响应。 我们假设它是通过解剖学 中线与脑干心脏结合核的连接 小脑为条件做出这些基本功能贡献 心动过缓。 与这个假设保持一致 目前的研究是为了进一步了解功能 小脑对自主条件的贡献通过研究 中线之间的解剖学和神经生理相互作用 小脑和蓬蒂纳派纳。 （PBN），脑干 心脏调节n。 （i）与 中线小脑A（（ii）直接解剖连接 前脑n。重要的是涉及条件性心动过缓，王牌。 朝向这一目标，目前的研究旨在（a） 准确确定中线的解剖学关系 小脑和PBN，（b）表征神经生理的反应 这些区域中神经元之间的相互作用以及改变 这可能是由于厌恶性帕夫洛维亚调节而发生的，（c） 成分的神经毒性病变的影响 小脑 - 寄生虫在获取和保留方面 在兔子中有条件性心动过缓。 这些数据将大大扩展我们对方式的了解 小脑神经元在哪些过程中与其他大脑区域相互作用 有条件的心动过缓，并增强我们对大脑功能的理解 通过更准确地识别参与的特定大脑系统 学习和记忆。 这样的标识是必要的先决条件 更完整地了解超微结构和分子 学习和记忆的基板；流程基于生存的基础 生物。 鉴于最近的证据表明，这些数据将令人兴奋 小脑参与复杂的人类学习和情感 过程。