DEVELOPMENT OF NEUROMODULATION IN SENSORY NEURONS

感觉神经元神经调节的发展

• 批准号: 3477081
• 负责人: Angeles Badell Ribera
• 金额: $ 7.69万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-25 至 1990-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3477081
• 关键词: G protein; Xenopus; action potentials; adenylate cyclase; autoradiography; binding proteins; calmodulin; cell age; cyclic AMP; cyclic GMP; dorsal root; electrophysiology; enkephalins; fresh water environment; gamma aminobutyrate; guanosine triphosphate; immature animal; lipid metabolism; membrane potentials; neurotransmitters; phosphatidylinositols; phosphorylation; protein kinase C; serotonin; single cell analysis; spinal ganglion; tritium

This proposal addresses questions about the development of modulation by neurotransmitters of the action potential of dorsal root ganglion cells of Xenopus tadpoles. My goals are (a) to determine the extent of modulation by GABA, serotonin or enkephalin of the action potential of dorsal root ganglion cells, (b) to determine the timing of the acquisition of neuromodulation by examining the correleation of neuromodulation with (i) the type of action potential expressed by the neuron, (ii) the age of the cell examined, (iii) the developmental stage of the tadpole, (c) to identify the membrane current that is sensitive to modulation, and (d) to investigate the mechanism of this modulation with respect both to developmental properties and roles of likely molecular intracellular components (GTP binding proteins, protein phosphorylation). Blockers and stimulators of these regulatory mechanisms will be used. The studies will be carried out on in vivo preparations of Xenopus tadpoles over a range of ages. Intracellular recording under current clamp conditions will be used to measure membrane potentials, action potentials, input resistance, and membrane potential and conductance changes in response to application of neurotransmitter. The effects of modulators on individual membrane currents (e.g., Na+, Ca2+, or K+ current) from neurons whose intracellular contents from cell- attached patches will be recorded using patch clamp techniques. Cell birthdating will be accomplished by 3H-thymidine labeling and autoradiographic analysis of dorsal root ganglion cells in Xenopus tadpoles whose exposures to label are begun at different stages between 1.5 and 45 days of embryonic development.

该提案解决了有关发展的问题 神经递质对背侧动作电位的调节 非洲爪蟾蝌蚪的根神经节细胞。 我的目标是 (a) 确定 GABA、血清素或的调节程度 脑啡肽对背根神经节细胞动作电位的影响，(b) 确定获得神经调节的时间 检查神经调节与 (i) 类型的相关性 神经元表达的动作电位，(ii) 细胞的年龄 检查，(iii) 蝌蚪的发育阶段，(c) 识别对调制敏感的膜电流， (d) 研究这种调制的机制 尊重可能的发展特性和角色 细胞内分子成分（GTP 结合蛋白、蛋白质 磷酸化）。 这些监管的阻碍者和刺激者 将使用机制。 研究将在 不同年龄段爪蟾蝌蚪的体内制剂。 当前钳条件下的细胞内记录将是 用于测量膜电位、动作电位、输入 电阻、膜电位​​和电导的变化 对神经递质应用的反应。 的影响 单个膜电流的调节剂（例如 Na+、Ca2+ 或 K+电流）来自神经元，其细胞内内容物来自细胞- 将使用膜片钳技术记录附加的膜片。 细胞生日将通过 3H-胸苷标记来完成 背根神经节细胞的放射自显影分析 非洲爪蟾蝌蚪暴露于标签的时间是不同的 胚胎发育1.5至45天的阶段。